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- W3201847411 abstract "The study describes the features of the kanamycin application for the effective selection of transgenic poplar expressing nptII gene. It has been shown that kanamycin concentration of 50 mg L−1 in the nutrient medium is the most suitable concentration for the effective selection of transgenic Berlin poplar plants for both regeneration and rooting stages. At the same time, 50 mg L−1 of kanamycin in the nutrient medium may result in the loss of the less resistant transgenic plants, so lower concentrations may be also applied. Kanamycin concentrations of 25 and 35 mg L−1 can give a false positive signal of transgenesis. Transgenic poplars expressing nptII gene can be effectively regenerated on nutrient media with kanamycin at concentrations up to 100 mg L−1." @default.
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- W3201847411 date "2021-09-01" @default.
- W3201847411 modified "2023-09-24" @default.
- W3201847411 title "Kanamycin application features for the selective screening of genetically modified Populus × berolinensis" @default.
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- W3201847411 doi "https://doi.org/10.1088/1755-1315/839/4/042020" @default.
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