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- W3201907368 abstract "Roti is one of the most farmer preferred cassava local genotype in Indonesia regarding its good taste. Embryogenic callus induction of Roti is critical to be established for many purposes such as plant propagation and genetic engineering. The research was conducted in Laboratory of Plant Molecular Genetic and Biosynthetic Pathway Alteration, Research Centre for Biotechnology, Indonesian Institute of Sciences (LIPI). The methods were divided into shoots induction from cuttings grown in the green house and embryogenic callus induction by using leaf lobes as initial explants. The media formulation used for callus induction were (1) MS 4 persen + 5�M CuSO4 + 10 mg/L Picloram, (2) MS 4 persen + 10 �M CuSO4 + 10 mg/L Picloram, and (3) MS 4 persen + 2 �M CuSO4 + 18 mg/L Picloram + 1 mM Glutamine. Callus could be produced from explants on all media following three to four weeks induction. However, only that cultured on media containing glutamine could produce embryogenic callus. The optimum medium for embryogenic callus production of Roti was MS 4 persen + 2 �M CUSO4 + 18 mg/L Picloram + 1 mM Glutamine, with 88.2 persen efficiency. Adira 4 which was known as a responsive variety was compared to determine the responsiveness of �Roti� in terms of embryogenic callus production. Embryogenic callus obtained is currently being used as materials for genetic transformation. The procedure established for �Roti� would open up the improvement of local genotype especially that preferred by farmers, industries and other users after improve for wider uses." @default.
- W3201907368 created "2021-10-11" @default.
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- W3201907368 date "2012-01-01" @default.
- W3201907368 modified "2023-09-27" @default.
- W3201907368 title "THE SUPERIORITY OF GLUTAMINE IN EMBRYOGENIC CALLUS INDUCTION OF ROTI CASSAVA FARMER PREFERENCE GENOTYPE" @default.
- W3201907368 hasPublicationYear "2012" @default.
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