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- W3201993991 abstract "Abstract Glycoside hydrolase family1 β-glucosidases play a variety of roles in plants, but their in planta functions are largely unknown in rice (Oryza sativa). In this study, the biological function of Os12BGlu38, a rice β-glucosidase, expressed in bicellular to mature pollen, was examined. Genotype analysis of progeny of the self-fertilized heterozygous Os12BGlu38 T-DNA mutant, os12bglu38-1, found no homozygotes and a 1:1 ratio of wild type to heterozygotes. Reciprocal cross analysis demonstrated that Os12BGlu38 deficiency cannot be inherited through the male gamete. In cytological analysis, the mature mutant pollen appeared shrunken and empty. Histochemical staining and TEM showed that mutant pollen lacked intine cell wall, which was rescued by introduction of wild-type Os12BGlu38 genomic DNA. Metabolite profiling analysis revealed that cutin monomers and waxes, the components of the pollen exine layer, were increased in anthers carrying pollen of os12bglu38-1 compared with wild type and complemented lines. Os12BGlu38 fused with green fluorescent protein was localized to the plasma membrane in rice and tobacco. Recombinant Os12BGlu38 exhibited β-glucosidase activity on the universal substrate p-nitrophenyl β-d-glucoside and some oligosaccharides and glycosides. These findings provide evidence that function of a plasma membrane-associated β-glucosidase is necessary for proper intine development." @default.
- W3201993991 created "2021-10-11" @default.
- W3201993991 creator A5007370787 @default.
- W3201993991 creator A5031470544 @default.
- W3201993991 creator A5058785718 @default.
- W3201993991 creator A5061095593 @default.
- W3201993991 creator A5064122653 @default.
- W3201993991 creator A5072698520 @default.
- W3201993991 creator A5082772780 @default.
- W3201993991 creator A5085788048 @default.
- W3201993991 creator A5090059241 @default.
- W3201993991 creator A5090619385 @default.
- W3201993991 date "2021-09-27" @default.
- W3201993991 modified "2023-10-17" @default.
- W3201993991 title "Rice β-glucosidase Os12BGlu38 is required for synthesis of intine cell wall and pollen fertility" @default.
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- W3201993991 doi "https://doi.org/10.1093/jxb/erab439" @default.