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- W3202000151 abstract "ABSTRACT Cell-cell interactions govern differentiation and cell competition in pluripotent cells during early development, but the investigation of such processes is hindered by a lack of efficient analysis tools. Here we introduce SyNPL: clonal pluripotent stem cell lines which employ optimised Synthetic Notch (SynNotch) technology to report cell-cell interactions between engineered “sender” and “receiver” cells in cultured pluripotent cells and chimaeric mouse embryos. A modular design makes it straightforward to adapt the system for programming differentiation decisions non-cell-autonomously in receiver cells in response to direct contact with sender cells. We demonstrate the utility of this system by enforcing neuronal differentiation at the boundary between two cell populations. In summary, we provide a new tool which could be used to identify cell interactions and to profile changes in gene or protein expression that result from direct cell-cell contact with defined cell populations in culture and in early embryos, and which can be adapted to generate synthetic patterning of cell fate decisions. SUMMARY STATEMENT Optimised Synthetic Notch circuitry in mouse pluripotent stem cells provides a modular tool to monitor cell-cell interactions and program synthetic patterning of cell fates in culture and in embryos." @default.
- W3202000151 created "2021-10-11" @default.
- W3202000151 creator A5016654053 @default.
- W3202000151 creator A5037431611 @default.
- W3202000151 creator A5037675499 @default.
- W3202000151 creator A5045038652 @default.
- W3202000151 creator A5050015299 @default.
- W3202000151 creator A5081766414 @default.
- W3202000151 date "2021-09-24" @default.
- W3202000151 modified "2023-10-06" @default.
- W3202000151 title "SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions <i>in vitro</i> and <i>in vivo</i>" @default.
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