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- W3202079308 endingPage "10987" @default.
- W3202079308 startingPage "10975" @default.
- W3202079308 abstract "The interaction of transcription factors with their response elements in DNA is emerging as a highly complex process, whose characterization requires measuring the full distribution of binding and dissociation times in a well-controlled assay. Here, we present a single-molecule assay that exploits the thermal fluctuations of a DNA hairpin to detect the association and dissociation of individual, unlabeled transcription factors. We demonstrate this new approach by following the binding of Egr1 to its consensus motif and the three binding sites found in the promoter of the Lhb gene, and find that both association and dissociation are modulated by the 9 bp core motif and the sequences around it. In addition, CpG methylation modulates the dissociation kinetics in a sequence and position-dependent manner, which can both stabilize or destabilize the complex. Together, our findings show how variations in sequence and methylation patterns synergistically extend the spectrum of a protein's binding properties, and demonstrate how the proposed approach can provide new insights on the function of transcription factors." @default.
- W3202079308 created "2021-10-11" @default.
- W3202079308 creator A5017395403 @default.
- W3202079308 creator A5055748555 @default.
- W3202079308 creator A5069205681 @default.
- W3202079308 creator A5075596604 @default.
- W3202079308 date "2021-10-04" @default.
- W3202079308 modified "2023-10-14" @default.
- W3202079308 title "Single molecule characterization of the binding kinetics of a transcription factor and its modulation by DNA sequence and methylation" @default.
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- W3202079308 doi "https://doi.org/10.1093/nar/gkab843" @default.
- W3202079308 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8565314" @default.
- W3202079308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34606618" @default.
- W3202079308 hasPublicationYear "2021" @default.