Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202201703> ?p ?o ?g. }
- W3202201703 endingPage "16319" @default.
- W3202201703 startingPage "16313" @default.
- W3202201703 abstract "Single-molecule measurements provide detailed mechanistic insights into molecular processes, for example in genome regulation where DNA access is controlled by nucleosomes and the chromatin machinery. However, real-time single-molecule observations of nuclear factors acting on defined chromatin substrates are challenging to perform quantitatively and reproducibly. Here we present XSCAN (multiplexed single-molecule detection of chromatin association), a method to parallelize single-molecule experiments by simultaneous imaging of a nucleosome library, where each nucleosome type carries an identifiable DNA sequence within its nucleosomal DNA. Parallel experiments are subsequently spatially decoded, via the detection of specific binding of dye-labeled DNA probes. We use this method to reveal how the Cas9 nuclease overcomes the nucleosome barrier when invading chromatinized DNA as a function of PAM position." @default.
- W3202201703 created "2021-10-11" @default.
- W3202201703 creator A5000173541 @default.
- W3202201703 creator A5014052655 @default.
- W3202201703 creator A5024361562 @default.
- W3202201703 creator A5039437314 @default.
- W3202201703 creator A5054204400 @default.
- W3202201703 creator A5060978585 @default.
- W3202201703 date "2021-10-01" @default.
- W3202201703 modified "2023-10-15" @default.
- W3202201703 title "Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor" @default.
- W3202201703 cites W1837454973 @default.
- W3202201703 cites W1970196890 @default.
- W3202201703 cites W1988997227 @default.
- W3202201703 cites W1995143570 @default.
- W3202201703 cites W2007730035 @default.
- W3202201703 cites W2010405881 @default.
- W3202201703 cites W2045435533 @default.
- W3202201703 cites W2050480410 @default.
- W3202201703 cites W2057586248 @default.
- W3202201703 cites W2074238305 @default.
- W3202201703 cites W2085258086 @default.
- W3202201703 cites W2087225474 @default.
- W3202201703 cites W2109281145 @default.
- W3202201703 cites W2129788878 @default.
- W3202201703 cites W2133582783 @default.
- W3202201703 cites W2141991853 @default.
- W3202201703 cites W2142844453 @default.
- W3202201703 cites W2144792857 @default.
- W3202201703 cites W2150881786 @default.
- W3202201703 cites W2151553287 @default.
- W3202201703 cites W2160051211 @default.
- W3202201703 cites W2177540144 @default.
- W3202201703 cites W2228397844 @default.
- W3202201703 cites W2237671266 @default.
- W3202201703 cites W2304268185 @default.
- W3202201703 cites W2342818826 @default.
- W3202201703 cites W2345911841 @default.
- W3202201703 cites W2523244706 @default.
- W3202201703 cites W2537157985 @default.
- W3202201703 cites W2612451420 @default.
- W3202201703 cites W2741989799 @default.
- W3202201703 cites W2783716336 @default.
- W3202201703 cites W2906655493 @default.
- W3202201703 cites W2922114087 @default.
- W3202201703 cites W2947339497 @default.
- W3202201703 cites W2952694437 @default.
- W3202201703 cites W2953019923 @default.
- W3202201703 cites W2970929986 @default.
- W3202201703 cites W2990228889 @default.
- W3202201703 cites W3119665747 @default.
- W3202201703 cites W3134237166 @default.
- W3202201703 cites W3161365429 @default.
- W3202201703 cites W3202079308 @default.
- W3202201703 doi "https://doi.org/10.1021/jacs.1c06195" @default.
- W3202201703 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8517959" @default.
- W3202201703 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34597515" @default.
- W3202201703 hasPublicationYear "2021" @default.
- W3202201703 type Work @default.
- W3202201703 sameAs 3202201703 @default.
- W3202201703 citedByCount "5" @default.
- W3202201703 countsByYear W32022017032022 @default.
- W3202201703 countsByYear W32022017032023 @default.
- W3202201703 crossrefType "journal-article" @default.
- W3202201703 hasAuthorship W3202201703A5000173541 @default.
- W3202201703 hasAuthorship W3202201703A5014052655 @default.
- W3202201703 hasAuthorship W3202201703A5024361562 @default.
- W3202201703 hasAuthorship W3202201703A5039437314 @default.
- W3202201703 hasAuthorship W3202201703A5054204400 @default.
- W3202201703 hasAuthorship W3202201703A5060978585 @default.
- W3202201703 hasBestOaLocation W32022017032 @default.
- W3202201703 hasConcept C12554922 @default.
- W3202201703 hasConcept C185592680 @default.
- W3202201703 hasConcept C193646685 @default.
- W3202201703 hasConcept C2777271071 @default.
- W3202201703 hasConcept C51679486 @default.
- W3202201703 hasConcept C552990157 @default.
- W3202201703 hasConcept C55493867 @default.
- W3202201703 hasConcept C70721500 @default.
- W3202201703 hasConcept C83640560 @default.
- W3202201703 hasConcept C84772758 @default.
- W3202201703 hasConcept C86803240 @default.
- W3202201703 hasConceptScore W3202201703C12554922 @default.
- W3202201703 hasConceptScore W3202201703C185592680 @default.
- W3202201703 hasConceptScore W3202201703C193646685 @default.
- W3202201703 hasConceptScore W3202201703C2777271071 @default.
- W3202201703 hasConceptScore W3202201703C51679486 @default.
- W3202201703 hasConceptScore W3202201703C552990157 @default.
- W3202201703 hasConceptScore W3202201703C55493867 @default.
- W3202201703 hasConceptScore W3202201703C70721500 @default.
- W3202201703 hasConceptScore W3202201703C83640560 @default.
- W3202201703 hasConceptScore W3202201703C84772758 @default.
- W3202201703 hasConceptScore W3202201703C86803240 @default.
- W3202201703 hasFunder F4320306082 @default.
- W3202201703 hasFunder F4320309597 @default.
- W3202201703 hasFunder F4320320915 @default.
- W3202201703 hasFunder F4320320924 @default.
- W3202201703 hasFunder F4320338335 @default.