FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202343645> ?p ?o ?g. }

• W3202343645 abstract "Indonesia is the largest consumer of rice in the world. International Rice Research Institute (IRRI) estimated that Indonesia may require an increase in rice production for the next 25 years. Among many constrains in rice production increement is plant diseases including tungro and grassy stunt diseases. Tungro disease is caused by infection of two viruses, Rice tungro bacilliform tungrovirus (RTBV) and Rice tungro spherical waikavirus (RTSV). These viruses are transmitted by the rice leaf hopper vectors which mainly is green leafhoppers (Nephotettix virescens). RTBV is a determinant of the disease symptoms eventhough it could not infect rice without the presence of RTSV. Infected plants showed typical tungro symptoms involving stunting, yellow or orange-yellow discoloration of the leaves, reduced number of tillers and rust-colored spots on the leaves. In 2006 a new symptoms was found in paddy crop in Indonesia, i.e. yellow-orange discoloration of the leaves, reduced number of tillers without any reduction in plant height. It was reported that the disease is caused by infection of Rice grassy stunt tenuivirus (RGSV) the cause of grassy stunt disease. Symptoms similarity of tungro and RGSV infection may cause difficulty in disease diagnosis and cause confusion in determining diseases control strategies. Therefore research on symptoms and genetic diversity of RTBV and RGSV is necessary to determine the appropriate control strategy. The genetic diversity of RTBV isolates from South Asia and Southeast Asia as well as from some tungro endemic area in Indonesia based on the symptoms variation and diversity of coat protein gene have been reported. The genetic diversity of the isolates RGSV from two areas in Taiwan and Philippines based on symptoms variation have also been reported. Genetic and symptoms diversity of RTBV and RGSV could be analysed through the diversity of coat protein gene. Coat protein gene is known as a determinant factor of infection, virulence, and symptoms induction. The research is conducted to study the diversity of symptoms and coat protein gene of RTBV and RGSV from several districts in Java. The areas in Bogor, Subang, Pandeglang, Klaten, Batang, Sleman, Jember and Banyuwangi were selected as disease survey location because these regions were reported as endemic areas for tungro disease and brown leafhopper. The study includes three main activities, i.e. observation of symptoms in the field and sampling of infected plants, detection of RTBV and RGSV, and analysis of diversity of coat protein gene of RTBV and RGSV. Most infected plants in the fields showed tungro-like symptoms. Two variations of symptoms can be classified by the number of tillers and plant height. These symptoms variations can be caused by RTBV or RGSV infection or mix infection of RTBV and RGSV as indicated by detection results using PCR. Identification of total 64 samples showed infection of RTBV in 13 samples, RGSV in 36 samples and mix of RTBV and RGSV in 2 samples. The results showed that tungro-like symptoms were positive from infection of RGSV. Therefore identification of diseases based only on symptoms are not sufficient. The whole coat protein gene of RTBV was amplified using DA-F and DA-R primers with the amplicon of 1224 bp. Part of coat protein gene of RGSV amplified using F3 and B3 primers with the amplicon of 243 bp. Amplified DNAs were then used for sequencing and further analysis of genetic diversity among isolates using phylogenetic analysis by Neighbor-Joining method. Analysis of nucleotide sequence of coat protein gene from 10 isolates of RTBV showed 40 persen to 100 persen similarity, whereas analysis of amino acid sequence of the isolates showed 88 persen to 100 persen similarity. Analysis of nucleotide sequence of coat protein gene from 20 isolates of RGSV showed 30 persen to 98 persen similarity, whereas analysis of amino acid sequence of the isolates showed 20 persen to 100 persen similarity. The sequence analysis indicated that genetic diversity of RTBV occurred only in nucleotide level, whereas genetic diversity of RGSV is high on nucleotide as well as amino acid level. Phylogenetic trees based on nucleotide sequence of coat protein gene showed that 10 isolates of RTBV can be differentiated into 3 groups whereas 20 isolates of RGSV can be differentiated into 4 groups. The diversity of RTBV and RGSV isolates from Java is not location specific. Genetic value between RTBV isolates from Java with isolates outside Indonesia explained that RTBV isolates from Java is similar strain with RTBV isolates that have been reported. Genetic distance‟s value between RGSV isolates from Java with isolates outside Indonesia explained that RGSV isolates from Java is different strain with RGSV isolates that have been reported. Further research is required to determine the genetic and symptoms diversity of RTBV and RGSV isolates from other tungro and grassy stunt endemic areas in Indonesia." @default.
• W3202343645 created "2021-10-11" @default.
• W3202343645 creator A5036488121 @default.
• W3202343645 creator A5053525927 @default.
• W3202343645 creator A5090944513 @default.
• W3202343645 date "2013-01-01" @default.
• W3202343645 modified "2023-09-26" @default.
• W3202343645 title "Keragaman Gen Protein Selubung Isolat Rice tungro bacilliform tungrovirus (RTBV) dan Rice grassy stunt tenuivirus (RGSV) dari beberapa Kabupaten di Pulau Jawa" @default.
• W3202343645 cites W1015313778 @default.
• W3202343645 cites W1483247593 @default.
• W3202343645 cites W1522583275 @default.
• W3202343645 cites W1542553009 @default.
• W3202343645 cites W1577683190 @default.
• W3202343645 cites W1578895149 @default.
• W3202343645 cites W1972065576 @default.
• W3202343645 cites W1984594263 @default.
• W3202343645 cites W1990966944 @default.
• W3202343645 cites W1993273300 @default.
• W3202343645 cites W2007252443 @default.
• W3202343645 cites W2008062133 @default.
• W3202343645 cites W2009900921 @default.
• W3202343645 cites W2011130803 @default.
• W3202343645 cites W2016728886 @default.
• W3202343645 cites W2030425354 @default.
• W3202343645 cites W2038676266 @default.
• W3202343645 cites W2043263599 @default.
• W3202343645 cites W2043264389 @default.
• W3202343645 cites W2055210379 @default.
• W3202343645 cites W2055750336 @default.
• W3202343645 cites W2057918551 @default.
• W3202343645 cites W2063613248 @default.
• W3202343645 cites W2066917814 @default.
• W3202343645 cites W2069677269 @default.
• W3202343645 cites W2073362325 @default.
• W3202343645 cites W2076320695 @default.
• W3202343645 cites W2086463133 @default.
• W3202343645 cites W2086690965 @default.
• W3202343645 cites W2106595860 @default.
• W3202343645 cites W2107549722 @default.
• W3202343645 cites W2108441780 @default.
• W3202343645 cites W2114552893 @default.
• W3202343645 cites W2118581693 @default.
• W3202343645 cites W2123397770 @default.
• W3202343645 cites W2124802250 @default.
• W3202343645 cites W2128341508 @default.
• W3202343645 cites W2135737180 @default.
• W3202343645 cites W2141558216 @default.
• W3202343645 cites W2148176142 @default.
• W3202343645 cites W2151193526 @default.
• W3202343645 cites W2158357683 @default.
• W3202343645 cites W2158680373 @default.
• W3202343645 cites W2164710927 @default.
• W3202343645 cites W2169571148 @default.
• W3202343645 cites W2187825829 @default.
• W3202343645 cites W2250675271 @default.
• W3202343645 cites W2292324250 @default.
• W3202343645 cites W243043770 @default.
• W3202343645 cites W2563882056 @default.
• W3202343645 cites W2799560457 @default.
• W3202343645 cites W54608552 @default.
• W3202343645 cites W2728446467 @default.
• W3202343645 hasPublicationYear "2013" @default.
• W3202343645 type Work @default.
• W3202343645 sameAs 3202343645 @default.
• W3202343645 citedByCount "0" @default.
• W3202343645 crossrefType "journal-article" @default.
• W3202343645 hasAuthorship W3202343645A5036488121 @default.
• W3202343645 hasAuthorship W3202343645A5053525927 @default.
• W3202343645 hasAuthorship W3202343645A5090944513 @default.
• W3202343645 hasConcept C137580998 @default.
• W3202343645 hasConcept C144027150 @default.
• W3202343645 hasConcept C42972112 @default.
• W3202343645 hasConcept C6557445 @default.
• W3202343645 hasConcept C71924100 @default.
• W3202343645 hasConcept C83082669 @default.
• W3202343645 hasConcept C86803240 @default.
• W3202343645 hasConceptScore W3202343645C137580998 @default.
• W3202343645 hasConceptScore W3202343645C144027150 @default.
• W3202343645 hasConceptScore W3202343645C42972112 @default.
• W3202343645 hasConceptScore W3202343645C6557445 @default.
• W3202343645 hasConceptScore W3202343645C71924100 @default.
• W3202343645 hasConceptScore W3202343645C83082669 @default.
• W3202343645 hasConceptScore W3202343645C86803240 @default.
• W3202343645 hasLocation W32023436451 @default.
• W3202343645 hasOpenAccess W3202343645 @default.
• W3202343645 hasPrimaryLocation W32023436451 @default.
• W3202343645 isParatext "false" @default.
• W3202343645 isRetracted "false" @default.
• W3202343645 magId "3202343645" @default.
• W3202343645 workType "article" @default.