Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202360414> ?p ?o ?g. }
- W3202360414 endingPage "e27304" @default.
- W3202360414 startingPage "e27304" @default.
- W3202360414 abstract "Abstract Background: Osteosarcoma is one of the most common bone tumors, with a high degree of malignancy and a poor prognosis. Recent studies have shown that THZ2, a cyclin-dependent kinase 7 inhibitor, can exhibit strong antibone tumor effects in vivo and in vitro by inhibiting transcriptional activity. In this study, by screening the differentially expressed genes (DEGs) of osteosarcoma cells before and after THZ2 treatment, it provides new possible targets for the future targeted therapy of osteosarcoma. Methods: Download the gene expression profile of GSE134603 from the Gene Expression Omnibus database, and use the R software package “limma Geoquery” to screen DEGs. DAVID database was used for gene ontology analysis of DEGs. Use search tool for the retrieval of interacting genes online database and Cytoscape software to construct protein–protein interaction network. Use the “MCODE” plugin in Cytoscape to analyze key molecular complexes (module) of DEGs, and use the “Cluego” plugin to perform Kyoto Encyclopedia of Genes and Genomes enrichment analysis on module genes. The Hub gene is selected from the genes in DEGs that coexist in the top 30 Degree and the Kyoto Encyclopedia of Genes and Genomes pathway. Results: A total of 1033 DEGs were screened, including 800 up-regulated genes and 233 down-regulated genes. Gene ontology analysis showed that cell component is the main enrichment area of DEGs, mainly in the nucleus, cytoplasm, and nucleoplasm. In addition, in molecular function analysis, DEGs are mainly enriched in the process of protein binding. In biological process analysis, changes in DEGs can also be observed in transcription and regulation using DNA as a template. Twenty-nine module genes are enriched in the Ribosome biogenesis in eukaryotes pathway. Finally, 4 key genes are drawn: essential for mitotic growth 1, U3 SnoRNP protein 3 homolog, U3 small nucleolar RNA-associated protein 15 homolog, and WD repeat domain 3. Conclusion: This study found that the 4 genes essential for mitotic growth 1, U3 SnoRNP protein 3 homolog, U3 small nucleolar RNA-associated protein 15 homolog, WD repeat domain 3, and the ribosome biogenesis in eukaryotes pathway play a very important role in the occurrence and development of osteosarcoma, and can become a new target for molecular targeted therapy of osteosarcoma in the future." @default.
- W3202360414 created "2021-10-11" @default.
- W3202360414 creator A5000012587 @default.
- W3202360414 creator A5009927370 @default.
- W3202360414 creator A5017841299 @default.
- W3202360414 creator A5056608424 @default.
- W3202360414 creator A5070066511 @default.
- W3202360414 creator A5083045131 @default.
- W3202360414 creator A5087035669 @default.
- W3202360414 creator A5089482025 @default.
- W3202360414 creator A5089586811 @default.
- W3202360414 date "2021-10-01" @default.
- W3202360414 modified "2023-10-16" @default.
- W3202360414 title "Identification of key genes in osteosarcoma – before and after CDK7 treatment" @default.
- W3202360414 cites W1513821330 @default.
- W3202360414 cites W1519934593 @default.
- W3202360414 cites W1975294927 @default.
- W3202360414 cites W1980088710 @default.
- W3202360414 cites W1982918697 @default.
- W3202360414 cites W1988199805 @default.
- W3202360414 cites W1995071974 @default.
- W3202360414 cites W1995301112 @default.
- W3202360414 cites W2009659072 @default.
- W3202360414 cites W2013303176 @default.
- W3202360414 cites W2016309119 @default.
- W3202360414 cites W2020920713 @default.
- W3202360414 cites W2036503673 @default.
- W3202360414 cites W2045080985 @default.
- W3202360414 cites W2049858249 @default.
- W3202360414 cites W2058427048 @default.
- W3202360414 cites W2060334131 @default.
- W3202360414 cites W2069174158 @default.
- W3202360414 cites W2071785498 @default.
- W3202360414 cites W2072684537 @default.
- W3202360414 cites W2086591998 @default.
- W3202360414 cites W2091408887 @default.
- W3202360414 cites W2107929386 @default.
- W3202360414 cites W2118258530 @default.
- W3202360414 cites W2127748864 @default.
- W3202360414 cites W2128125459 @default.
- W3202360414 cites W2139619092 @default.
- W3202360414 cites W2143284374 @default.
- W3202360414 cites W2146512944 @default.
- W3202360414 cites W2149765709 @default.
- W3202360414 cites W2158217645 @default.
- W3202360414 cites W2159675211 @default.
- W3202360414 cites W2169589032 @default.
- W3202360414 cites W2329719897 @default.
- W3202360414 cites W2346520135 @default.
- W3202360414 cites W2383217883 @default.
- W3202360414 cites W2560556363 @default.
- W3202360414 cites W2609470176 @default.
- W3202360414 cites W2620510751 @default.
- W3202360414 cites W2773619385 @default.
- W3202360414 cites W2969214885 @default.
- W3202360414 cites W2999161782 @default.
- W3202360414 cites W4246742253 @default.
- W3202360414 doi "https://doi.org/10.1097/md.0000000000027304" @default.
- W3202360414 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8483848" @default.
- W3202360414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34596127" @default.
- W3202360414 hasPublicationYear "2021" @default.
- W3202360414 type Work @default.
- W3202360414 sameAs 3202360414 @default.
- W3202360414 citedByCount "0" @default.
- W3202360414 crossrefType "journal-article" @default.
- W3202360414 hasAuthorship W3202360414A5000012587 @default.
- W3202360414 hasAuthorship W3202360414A5009927370 @default.
- W3202360414 hasAuthorship W3202360414A5017841299 @default.
- W3202360414 hasAuthorship W3202360414A5056608424 @default.
- W3202360414 hasAuthorship W3202360414A5070066511 @default.
- W3202360414 hasAuthorship W3202360414A5083045131 @default.
- W3202360414 hasAuthorship W3202360414A5087035669 @default.
- W3202360414 hasAuthorship W3202360414A5089482025 @default.
- W3202360414 hasAuthorship W3202360414A5089586811 @default.
- W3202360414 hasBestOaLocation W32023604141 @default.
- W3202360414 hasConcept C104317684 @default.
- W3202360414 hasConcept C141231307 @default.
- W3202360414 hasConcept C150194340 @default.
- W3202360414 hasConcept C152724338 @default.
- W3202360414 hasConcept C18431079 @default.
- W3202360414 hasConcept C2777760704 @default.
- W3202360414 hasConcept C2987395477 @default.
- W3202360414 hasConcept C502942594 @default.
- W3202360414 hasConcept C54355233 @default.
- W3202360414 hasConcept C60644358 @default.
- W3202360414 hasConcept C70721500 @default.
- W3202360414 hasConcept C86803240 @default.
- W3202360414 hasConceptScore W3202360414C104317684 @default.
- W3202360414 hasConceptScore W3202360414C141231307 @default.
- W3202360414 hasConceptScore W3202360414C150194340 @default.
- W3202360414 hasConceptScore W3202360414C152724338 @default.
- W3202360414 hasConceptScore W3202360414C18431079 @default.
- W3202360414 hasConceptScore W3202360414C2777760704 @default.
- W3202360414 hasConceptScore W3202360414C2987395477 @default.
- W3202360414 hasConceptScore W3202360414C502942594 @default.
- W3202360414 hasConceptScore W3202360414C54355233 @default.
- W3202360414 hasConceptScore W3202360414C60644358 @default.
- W3202360414 hasConceptScore W3202360414C70721500 @default.