FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202508672> ?p ?o ?g. }

• W3202508672 endingPage "10519" @default.
• W3202508672 startingPage "10519" @default.
• W3202508672 abstract "IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H2O2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H2O2 or hypoxia (p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation (p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP, ERN1, HIF1A, and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation." @default.
• W3202508672 created "2021-10-11" @default.
• W3202508672 creator A5005416741 @default.
• W3202508672 creator A5007672077 @default.
• W3202508672 creator A5013385377 @default.
• W3202508672 creator A5043593241 @default.
• W3202508672 creator A5045720408 @default.
• W3202508672 creator A5059202198 @default.
• W3202508672 creator A5064858467 @default.
• W3202508672 creator A5072097397 @default.
• W3202508672 creator A5075544614 @default.
• W3202508672 creator A5084277079 @default.
• W3202508672 creator A5086374359 @default.
• W3202508672 date "2021-09-29" @default.
• W3202508672 modified "2023-10-15" @default.
• W3202508672 title "ROS/TNF-α Crosstalk Triggers the Expression of IL-8 and MCP-1 in Human Monocytic THP-1 Cells via the NF-κB and ERK1/2 Mediated Signaling" @default.
• W3202508672 cites W1439605962 @default.
• W3202508672 cites W1510258932 @default.
• W3202508672 cites W1739650398 @default.
• W3202508672 cites W1764140709 @default.
• W3202508672 cites W18347160 @default.
• W3202508672 cites W1958860490 @default.
• W3202508672 cites W1970333748 @default.
• W3202508672 cites W1974577462 @default.
• W3202508672 cites W1979454503 @default.
• W3202508672 cites W1983961700 @default.
• W3202508672 cites W1984583584 @default.
• W3202508672 cites W1988449390 @default.
• W3202508672 cites W1990088861 @default.
• W3202508672 cites W1992914650 @default.
• W3202508672 cites W1994851844 @default.
• W3202508672 cites W1997834526 @default.
• W3202508672 cites W1999433383 @default.
• W3202508672 cites W2002888398 @default.
• W3202508672 cites W2003270319 @default.
• W3202508672 cites W2007424363 @default.
• W3202508672 cites W2011035339 @default.
• W3202508672 cites W2019879894 @default.
• W3202508672 cites W2022801078 @default.
• W3202508672 cites W2034054192 @default.
• W3202508672 cites W2035104435 @default.
• W3202508672 cites W2036852690 @default.
• W3202508672 cites W2038151530 @default.
• W3202508672 cites W2038561084 @default.
• W3202508672 cites W2039620761 @default.
• W3202508672 cites W2044269687 @default.
• W3202508672 cites W2065892131 @default.
• W3202508672 cites W2067218278 @default.
• W3202508672 cites W2068133291 @default.
• W3202508672 cites W2074023056 @default.
• W3202508672 cites W2074098186 @default.
• W3202508672 cites W2077026831 @default.
• W3202508672 cites W2081282903 @default.
• W3202508672 cites W2093705034 @default.
• W3202508672 cites W2096384870 @default.
• W3202508672 cites W2097017740 @default.
• W3202508672 cites W2100316337 @default.
• W3202508672 cites W2106925175 @default.
• W3202508672 cites W2112573480 @default.
• W3202508672 cites W2122638636 @default.
• W3202508672 cites W2126269511 @default.
• W3202508672 cites W2129316761 @default.
• W3202508672 cites W2135410441 @default.
• W3202508672 cites W2135723092 @default.
• W3202508672 cites W2139923937 @default.
• W3202508672 cites W2143016221 @default.
• W3202508672 cites W2151672477 @default.
• W3202508672 cites W2165201727 @default.
• W3202508672 cites W2167067091 @default.
• W3202508672 cites W2167435864 @default.
• W3202508672 cites W2169313407 @default.
• W3202508672 cites W2170429710 @default.
• W3202508672 cites W2286962687 @default.
• W3202508672 cites W2461952548 @default.
• W3202508672 cites W2580419588 @default.
• W3202508672 cites W2727374696 @default.
• W3202508672 cites W2749248168 @default.
• W3202508672 cites W2789275310 @default.
• W3202508672 cites W2805693022 @default.
• W3202508672 cites W2912917749 @default.
• W3202508672 cites W4241216156 @default.
• W3202508672 cites W4254763216 @default.
• W3202508672 cites W4255292115 @default.
• W3202508672 doi "https://doi.org/10.3390/ijms221910519" @default.
• W3202508672 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8508672" @default.
• W3202508672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34638857" @default.
• W3202508672 hasPublicationYear "2021" @default.
• W3202508672 type Work @default.
• W3202508672 sameAs 3202508672 @default.
• W3202508672 citedByCount "24" @default.
• W3202508672 countsByYear W32025086722021 @default.
• W3202508672 countsByYear W32025086722022 @default.
• W3202508672 countsByYear W32025086722023 @default.
• W3202508672 crossrefType "journal-article" @default.
• W3202508672 hasAuthorship W3202508672A5005416741 @default.
• W3202508672 hasAuthorship W3202508672A5007672077 @default.
• W3202508672 hasAuthorship W3202508672A5013385377 @default.
• W3202508672 hasAuthorship W3202508672A5043593241 @default.

• next