Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202562012> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3202562012 endingPage "6927" @default.
- W3202562012 startingPage "6917" @default.
- W3202562012 abstract "Quantitative measure of a drug and its associated metabolite(s) with single-cell resolution is often limited by sampling throughput or other compromises that limit broad use. Here, we demonstrate the use of single-cell printing-liquid vortex capture-mass spectrometry (SCP-LVC-MS) to quantitatively measure the intracellular concentrations of amiodarone (AMIO) and its metabolite, N-desethylamiodarone (NDEA), from thousands of single cells across several AMIO incubation concentrations ranging from 0 to 10 μM. Concentrations obtained by SCP-LVC-MS were validated through comparison with average assays and traditional measurement of cells in bulk. Average of SCP-LVC-MS measurements and aggregate vial collection assay the concentrations differed by < 5%. Both AMIO and NDEA had clear log-normal distributions with similar standard deviation of concentrations in the cell population. The mean of both AMIO and NDEA intracellular concentrations were positively correlated with AMIO incubation concentration, increasing from 0.026 to 0.520 and 0.0055 to 0.048 mM for AMIO and NDEA, respectively. The standard deviation of AMIO and NDEA log-normal distribution fits were relatively similar in value across incubation concentrations, 0.15–0.19 log10 (mM), and exhibited a linear trend with respect to each other. The single cell-resolved conversion ratio of AMIO to NDEA increased with decreasing incubation concentration, 7 ± 2%, 18 ± 3%, and 20 ± 7% for 10.0, 1.0, and 0.1 μM AMIO incubation concentrations, respectively. Association with simultaneously measured lipids had several ions with statistically significant difference in intensity but no clear correlations with AMIO intracellular content was observed." @default.
- W3202562012 created "2021-10-11" @default.
- W3202562012 creator A5061041928 @default.
- W3202562012 creator A5082034283 @default.
- W3202562012 date "2021-10-01" @default.
- W3202562012 modified "2023-09-27" @default.
- W3202562012 title "Quantitation of amiodarone and N-desethylamiodarone in single HepG2 cells by single-cell printing-liquid vortex capture-mass spectrometry" @default.
- W3202562012 cites W1114793879 @default.
- W3202562012 cites W1963170190 @default.
- W3202562012 cites W1974759182 @default.
- W3202562012 cites W1997023648 @default.
- W3202562012 cites W2049554068 @default.
- W3202562012 cites W2050841253 @default.
- W3202562012 cites W2054014227 @default.
- W3202562012 cites W2060886568 @default.
- W3202562012 cites W2105976216 @default.
- W3202562012 cites W2111510713 @default.
- W3202562012 cites W2122518772 @default.
- W3202562012 cites W2123070092 @default.
- W3202562012 cites W2139104233 @default.
- W3202562012 cites W2152997281 @default.
- W3202562012 cites W2154335109 @default.
- W3202562012 cites W2177577974 @default.
- W3202562012 cites W2320185681 @default.
- W3202562012 cites W2334323124 @default.
- W3202562012 cites W2342511248 @default.
- W3202562012 cites W2401855417 @default.
- W3202562012 cites W2511297787 @default.
- W3202562012 cites W2522291185 @default.
- W3202562012 cites W2614820090 @default.
- W3202562012 cites W2749555181 @default.
- W3202562012 cites W2779023477 @default.
- W3202562012 cites W2805177864 @default.
- W3202562012 cites W2885741108 @default.
- W3202562012 cites W2888492955 @default.
- W3202562012 cites W2897589467 @default.
- W3202562012 cites W2937542470 @default.
- W3202562012 cites W2949769764 @default.
- W3202562012 cites W2990317030 @default.
- W3202562012 cites W3112983904 @default.
- W3202562012 cites W3120253918 @default.
- W3202562012 cites W421784205 @default.
- W3202562012 doi "https://doi.org/10.1007/s00216-021-03652-6" @default.
- W3202562012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34595558" @default.
- W3202562012 hasPublicationYear "2021" @default.
- W3202562012 type Work @default.
- W3202562012 sameAs 3202562012 @default.
- W3202562012 citedByCount "1" @default.
- W3202562012 countsByYear W32025620122023 @default.
- W3202562012 crossrefType "journal-article" @default.
- W3202562012 hasAuthorship W3202562012A5061041928 @default.
- W3202562012 hasAuthorship W3202562012A5082034283 @default.
- W3202562012 hasBestOaLocation W32025620122 @default.
- W3202562012 hasConcept C113196181 @default.
- W3202562012 hasConcept C119128265 @default.
- W3202562012 hasConcept C143701427 @default.
- W3202562012 hasConcept C1491633281 @default.
- W3202562012 hasConcept C162356407 @default.
- W3202562012 hasConcept C185592680 @default.
- W3202562012 hasConcept C25642318 @default.
- W3202562012 hasConcept C2776950831 @default.
- W3202562012 hasConcept C2777477808 @default.
- W3202562012 hasConcept C43617362 @default.
- W3202562012 hasConcept C55493867 @default.
- W3202562012 hasConcept C79879829 @default.
- W3202562012 hasConceptScore W3202562012C113196181 @default.
- W3202562012 hasConceptScore W3202562012C119128265 @default.
- W3202562012 hasConceptScore W3202562012C143701427 @default.
- W3202562012 hasConceptScore W3202562012C1491633281 @default.
- W3202562012 hasConceptScore W3202562012C162356407 @default.
- W3202562012 hasConceptScore W3202562012C185592680 @default.
- W3202562012 hasConceptScore W3202562012C25642318 @default.
- W3202562012 hasConceptScore W3202562012C2776950831 @default.
- W3202562012 hasConceptScore W3202562012C2777477808 @default.
- W3202562012 hasConceptScore W3202562012C43617362 @default.
- W3202562012 hasConceptScore W3202562012C55493867 @default.
- W3202562012 hasConceptScore W3202562012C79879829 @default.
- W3202562012 hasIssue "28" @default.
- W3202562012 hasLocation W32025620121 @default.
- W3202562012 hasLocation W32025620122 @default.
- W3202562012 hasLocation W32025620123 @default.
- W3202562012 hasOpenAccess W3202562012 @default.
- W3202562012 hasPrimaryLocation W32025620121 @default.
- W3202562012 hasRelatedWork W1534420425 @default.
- W3202562012 hasRelatedWork W2003174742 @default.
- W3202562012 hasRelatedWork W2003362673 @default.
- W3202562012 hasRelatedWork W2018070089 @default.
- W3202562012 hasRelatedWork W2042655533 @default.
- W3202562012 hasRelatedWork W2252451298 @default.
- W3202562012 hasRelatedWork W2287994681 @default.
- W3202562012 hasRelatedWork W2362213018 @default.
- W3202562012 hasRelatedWork W29133270 @default.
- W3202562012 hasRelatedWork W4233893874 @default.
- W3202562012 hasVolume "413" @default.
- W3202562012 isParatext "false" @default.
- W3202562012 isRetracted "false" @default.
- W3202562012 magId "3202562012" @default.
- W3202562012 workType "article" @default.