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• W3202673731 abstract "Abstract In this study, a medical device made of surface microstructured bacterial cellulose was produced using cellulose-producing acetic acid bacteria wild-type strains in combination with guided assembly-based biolithography. The medical device aims at interfering with the cell's focal adhesion establishment and maturation around implantable devices placed in soft tissues by the symmetrical array on its surface. A total of 25 Komagataeibacter strains was evaluated over a three-step selection. In the first step, the ability of strains to produce a suitable bacterial cellulose layer with high production yield was examined, then nine strains, with a uniform and smooth layer of bacterial cellulose, were cultured in a custom-made silicone bioreactor and finally the characteristics of the symmetrical array of topographic features on the surface were analysed. Selected strains showed high inter and intra species variability in bacterial cellulose production. The devices obtained by K2G30, K1G4, DSM 46590 ( Komagataeibacter xylinus ), K2A8 ( Komagataeibacter sp. ) and DSM 15973 T ( Komagataeibacter sucrofermentas) strains were pouched-formed with hexagonal surface pattern required for reducing the formation of fibrotic tissue around devices, once they are implanted in soft tissues. Our findings revealed the effectiveness of the selected Komagataeibacter wild-type strains in producing surface microstructured bacterial cellulose pouches for making biomedical devices." @default.
• W3202673731 created "2021-10-11" @default.
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• W3202673731 date "2021-09-29" @default.
• W3202673731 modified "2023-10-18" @default.
• W3202673731 title "Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography" @default.
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• W3202673731 doi "https://doi.org/10.1038/s41598-021-98705-2" @default.
• W3202673731 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8481549" @default.
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