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• W3202777402 abstract "ABSTRACT In 2016, several reports emerged of fresh-market staked tomato plants with severe symptoms of pith necrosis and premature death in commercial cultivation areas in the states of Paraná and Minas Gerais, Brazil, which are similar to those caused by Pseudomonas corrugata. Four bacterial strains were isolated from infected tissue samples and characterized as Gram-negative, aerobic, and fluorescent on King’s B Medium. LOPAT tests were performed and the isolate UFU H120 was classified into group Vb (+ + − + −) and the isolates UFU H3, UFU H6, and UFU H21 into group Va (− + − + −). The pathogenicity of the isolates was confirmed by artificial inoculation on tomato plants of Santa Cruz type, cv. Kada Gigante, and Koch’s postulates were accomplished successfully. The isolates sequences of their 16S rRNA gene region were compared with those deposited in GenBank, the isolate UFU H120 aligning with Pseudomonas fluorescens (99,45% similarity) and the isolates UFU H3, UFU H6, and UFU H21 with Pseudomonas putida (99.2, 99.53 and 99.64% similarity, respectively). Notably, P. fluorescens and P. putida are most known as saprophytic bacteria normally present in the soil, although both of these species have already been reported to infect tomato plants in Italy. However, so far, there have not been any reports of such bacteria being phytopathogenic on tomato or any crop in Brazil. Thus, to our knowledge, this is the first report of pith necrosis of such occurrence in Brazil." @default.
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• W3202777402 date "2021-09-21" @default.
• W3202777402 modified "2023-09-27" @default.
• W3202777402 title "Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil" @default.
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• W3202777402 doi "https://doi.org/10.1590/s0102-0536-20210303" @default.
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