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- W3202865718 abstract "This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Myocytes and non-myocytes extracted using this protocol are separated by centrifugation and/or fluorescence-activated cell sorting for use in downstream applications including single-cell omics or other bio-molecular analyses. For complete details on the use and execution of this protocol, please refer to McLellan et al. (2020)." @default.
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- W3202865718 date "2021-12-01" @default.
- W3202865718 modified "2023-10-16" @default.
- W3202865718 title "A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts" @default.
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- W3202865718 doi "https://doi.org/10.1016/j.xpro.2021.100866" @default.
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