Matches in SemOpenAlex for { <https://semopenalex.org/work/W3202893154> ?p ?o ?g. }
- W3202893154 abstract "Abstract Protoplast transient expression is a powerful strategy for gene functional characterization, especially in biochemical mechanism studies. We herein developed a highly efficient transient expression system for apple protoplasts. The abilities of the Arabidopsis thaliana and Malus domestica ubiquitin-10 ( AtUBQ10 and MdUBQ10 ) promoters to drive the expression of multiple genes were compared with that of the CaMV 35S promoter, and the results revealed that the AtUBQ10 and MdUBQ10 promoters were more efficient in apple protoplasts. With this system, we demonstrated that active AtMKK7ac could activate MAPK6/3/4 signaling cascades, which further regulated MdWRKY33 phosphorylation and stability in apple. Furthermore, the ligand-induced interaction between the immune receptor AtFLS2 and the coreceptor AtBAK1 was reconstituted in apple protoplasts. We also found that the stability of the bacterial effector AvrRpt2 was regulated by feedback involving auxin and the immune regulator RIN4. The system established herein will serve as a useful tool for the molecular and biochemical analyses of apple genes." @default.
- W3202893154 created "2021-10-11" @default.
- W3202893154 creator A5008015122 @default.
- W3202893154 creator A5016792966 @default.
- W3202893154 creator A5034887842 @default.
- W3202893154 creator A5054397784 @default.
- W3202893154 creator A5074121633 @default.
- W3202893154 creator A5082259017 @default.
- W3202893154 date "2021-10-01" @default.
- W3202893154 modified "2023-10-01" @default.
- W3202893154 title "Development of potent promoters that drive the efficient expression of genes in apple protoplasts" @default.
- W3202893154 cites W1654701538 @default.
- W3202893154 cites W1986135627 @default.
- W3202893154 cites W1990722409 @default.
- W3202893154 cites W2000821899 @default.
- W3202893154 cites W2007619416 @default.
- W3202893154 cites W2032240066 @default.
- W3202893154 cites W2034948175 @default.
- W3202893154 cites W2045733785 @default.
- W3202893154 cites W2048588293 @default.
- W3202893154 cites W2067519453 @default.
- W3202893154 cites W2092619415 @default.
- W3202893154 cites W2114615912 @default.
- W3202893154 cites W2123787026 @default.
- W3202893154 cites W2130990872 @default.
- W3202893154 cites W2138766417 @default.
- W3202893154 cites W2143577108 @default.
- W3202893154 cites W2147863419 @default.
- W3202893154 cites W2158215557 @default.
- W3202893154 cites W2159609199 @default.
- W3202893154 cites W2191141039 @default.
- W3202893154 cites W2331103978 @default.
- W3202893154 cites W2417670369 @default.
- W3202893154 cites W2578699590 @default.
- W3202893154 cites W2618402276 @default.
- W3202893154 cites W2800336448 @default.
- W3202893154 cites W2806550479 @default.
- W3202893154 cites W2807683374 @default.
- W3202893154 cites W2894946351 @default.
- W3202893154 cites W2898771822 @default.
- W3202893154 cites W2934650466 @default.
- W3202893154 cites W2952556634 @default.
- W3202893154 cites W2995345210 @default.
- W3202893154 cites W3019783516 @default.
- W3202893154 cites W3024449452 @default.
- W3202893154 cites W3028123991 @default.
- W3202893154 cites W3085196816 @default.
- W3202893154 cites W3113861263 @default.
- W3202893154 cites W3133795834 @default.
- W3202893154 doi "https://doi.org/10.1038/s41438-021-00646-4" @default.
- W3202893154 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8484340" @default.
- W3202893154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34593780" @default.
- W3202893154 hasPublicationYear "2021" @default.
- W3202893154 type Work @default.
- W3202893154 sameAs 3202893154 @default.
- W3202893154 citedByCount "11" @default.
- W3202893154 countsByYear W32028931542022 @default.
- W3202893154 countsByYear W32028931542023 @default.
- W3202893154 crossrefType "journal-article" @default.
- W3202893154 hasAuthorship W3202893154A5008015122 @default.
- W3202893154 hasAuthorship W3202893154A5016792966 @default.
- W3202893154 hasAuthorship W3202893154A5034887842 @default.
- W3202893154 hasAuthorship W3202893154A5054397784 @default.
- W3202893154 hasAuthorship W3202893154A5074121633 @default.
- W3202893154 hasAuthorship W3202893154A5082259017 @default.
- W3202893154 hasBestOaLocation W32028931541 @default.
- W3202893154 hasConcept C101762097 @default.
- W3202893154 hasConcept C103474210 @default.
- W3202893154 hasConcept C104317684 @default.
- W3202893154 hasConcept C143065580 @default.
- W3202893154 hasConcept C150194340 @default.
- W3202893154 hasConcept C165864922 @default.
- W3202893154 hasConcept C173749725 @default.
- W3202893154 hasConcept C2775988993 @default.
- W3202893154 hasConcept C2777306635 @default.
- W3202893154 hasConcept C2779491563 @default.
- W3202893154 hasConcept C51785407 @default.
- W3202893154 hasConcept C54355233 @default.
- W3202893154 hasConcept C59822182 @default.
- W3202893154 hasConcept C86803240 @default.
- W3202893154 hasConcept C8891405 @default.
- W3202893154 hasConcept C95444343 @default.
- W3202893154 hasConceptScore W3202893154C101762097 @default.
- W3202893154 hasConceptScore W3202893154C103474210 @default.
- W3202893154 hasConceptScore W3202893154C104317684 @default.
- W3202893154 hasConceptScore W3202893154C143065580 @default.
- W3202893154 hasConceptScore W3202893154C150194340 @default.
- W3202893154 hasConceptScore W3202893154C165864922 @default.
- W3202893154 hasConceptScore W3202893154C173749725 @default.
- W3202893154 hasConceptScore W3202893154C2775988993 @default.
- W3202893154 hasConceptScore W3202893154C2777306635 @default.
- W3202893154 hasConceptScore W3202893154C2779491563 @default.
- W3202893154 hasConceptScore W3202893154C51785407 @default.
- W3202893154 hasConceptScore W3202893154C54355233 @default.
- W3202893154 hasConceptScore W3202893154C59822182 @default.
- W3202893154 hasConceptScore W3202893154C86803240 @default.
- W3202893154 hasConceptScore W3202893154C8891405 @default.
- W3202893154 hasConceptScore W3202893154C95444343 @default.
- W3202893154 hasIssue "1" @default.
- W3202893154 hasLocation W32028931541 @default.