FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3203059724> ?p ?o ?g. }

• W3203059724 abstract "Plasmodium 18S rRNA is a sensitive biomarker for detecting Plasmodium infection in human blood. Dried blood spots (DBS) are a practical sample type for malaria field studies to collect, store, and transport large quantities of blood samples for diagnostic testing. Pooled testing is a common way to reduce reagent costs and labour. This study examined performance of the Plasmodium 18S rRNA biomarker assay for DBS, improved assay sensitivity for pooled samples, and created graphical user interface (GUI) programmes for facilitating optimal pooling.DBS samples of varied parasite densities from clinical specimens, Plasmodium falciparum in vitro culture, and P. falciparum Armored RNA® were tested using the Plasmodium 18S rRNA quantitative triplex reverse transcription polymerase chain reaction (qRT-PCR) assay and a simplified duplex assay. DBS sample precision, linearity, limit of detection (LoD) and stability at varied storage temperatures were evaluated. Novel GUIs were created to model two-stage hierarchy, square matrix, and three-stage hierarchy pooling strategies with samples of varying positivity rates and estimated test counts. Seventy-eight DBS samples from persons residing in endemic regions with sub-patent infections were tested in pools and deconvoluted to identify positive cases.Assay performance showed linearity for DBS from 4 × 107 to 5 × 102 parasites/mL with strong correlation to liquid blood samples (r2 > 0.96). There was a minor quantitative reduction in DBS rRNA copies/mL compared to liquid blood samples. Analytical sensitivity for DBS was estimated 5.3 log copies 18S rRNA/mL blood (28 estimated parasites/mL). Properly preserved DBS demonstrated minimal degradation of 18S rRNA when stored at ambient temperatures for one month. A simplified duplex qRT-PCR assay omitting the human mRNA target showed improved analytical sensitivity, 1 parasite/mL blood, and was optimized for pooling. Optimal pooling sizes varied depending on prevalence. A pilot DBS study of the two-stage hierarchy pooling scheme corroborated results previously determined by testing individual DBS.The Plasmodium 18S rRNA biomarker assay can be applied to DBS collected in field studies. The simplified Plasmodium qRT-PCR assay and GUIs have been established to provide efficient means to test large quantities of DBS samples." @default.
• W3203059724 created "2021-10-11" @default.
• W3203059724 creator A5033464032 @default.
• W3203059724 creator A5042688658 @default.
• W3203059724 creator A5047311353 @default.
• W3203059724 creator A5054620494 @default.
• W3203059724 creator A5070774961 @default.
• W3203059724 date "2021-10-07" @default.
• W3203059724 modified "2023-10-17" @default.
• W3203059724 title "Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies" @default.
• W3203059724 cites W1622422817 @default.
• W3203059724 cites W1948731308 @default.
• W3203059724 cites W1966459680 @default.
• W3203059724 cites W1975680434 @default.
• W3203059724 cites W1980712697 @default.
• W3203059724 cites W1995491956 @default.
• W3203059724 cites W2053311263 @default.
• W3203059724 cites W2053925702 @default.
• W3203059724 cites W2055812034 @default.
• W3203059724 cites W2065283536 @default.
• W3203059724 cites W2090865169 @default.
• W3203059724 cites W2106702045 @default.
• W3203059724 cites W2115837962 @default.
• W3203059724 cites W2126892686 @default.
• W3203059724 cites W2143908311 @default.
• W3203059724 cites W2148765503 @default.
• W3203059724 cites W2161290118 @default.
• W3203059724 cites W2161559787 @default.
• W3203059724 cites W2162092231 @default.
• W3203059724 cites W2185132500 @default.
• W3203059724 cites W2514915382 @default.
• W3203059724 cites W2517775577 @default.
• W3203059724 cites W2602965957 @default.
• W3203059724 cites W2613332809 @default.
• W3203059724 cites W2755674774 @default.
• W3203059724 cites W2801419612 @default.
• W3203059724 cites W2810221000 @default.
• W3203059724 cites W2902520845 @default.
• W3203059724 cites W2914807841 @default.
• W3203059724 cites W2929535294 @default.
• W3203059724 cites W2942487916 @default.
• W3203059724 cites W2943040812 @default.
• W3203059724 cites W2943318827 @default.
• W3203059724 cites W2944083118 @default.
• W3203059724 cites W2969268926 @default.
• W3203059724 cites W2992075042 @default.
• W3203059724 cites W3012959128 @default.
• W3203059724 cites W3016372722 @default.
• W3203059724 cites W3036757363 @default.
• W3203059724 doi "https://doi.org/10.1186/s12936-021-03907-8" @default.
• W3203059724 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8499573" @default.
• W3203059724 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34620192" @default.
• W3203059724 hasPublicationYear "2021" @default.
• W3203059724 type Work @default.
• W3203059724 sameAs 3203059724 @default.
• W3203059724 citedByCount "7" @default.
• W3203059724 countsByYear W32030597242022 @default.
• W3203059724 countsByYear W32030597242023 @default.
• W3203059724 crossrefType "journal-article" @default.
• W3203059724 hasAuthorship W3203059724A5033464032 @default.
• W3203059724 hasAuthorship W3203059724A5042688658 @default.
• W3203059724 hasAuthorship W3203059724A5047311353 @default.
• W3203059724 hasAuthorship W3203059724A5054620494 @default.
• W3203059724 hasAuthorship W3203059724A5070774961 @default.
• W3203059724 hasBestOaLocation W32030597241 @default.
• W3203059724 hasConcept C104317684 @default.
• W3203059724 hasConcept C159047783 @default.
• W3203059724 hasConcept C190612196 @default.
• W3203059724 hasConcept C203014093 @default.
• W3203059724 hasConcept C2775867548 @default.
• W3203059724 hasConcept C2776597655 @default.
• W3203059724 hasConcept C2778048844 @default.
• W3203059724 hasConcept C2778371730 @default.
• W3203059724 hasConcept C2781188995 @default.
• W3203059724 hasConcept C2781197716 @default.
• W3203059724 hasConcept C47698986 @default.
• W3203059724 hasConcept C54355233 @default.
• W3203059724 hasConcept C60644358 @default.
• W3203059724 hasConcept C67905577 @default.
• W3203059724 hasConcept C70721500 @default.
• W3203059724 hasConcept C86803240 @default.
• W3203059724 hasConcept C90856448 @default.
• W3203059724 hasConceptScore W3203059724C104317684 @default.
• W3203059724 hasConceptScore W3203059724C159047783 @default.
• W3203059724 hasConceptScore W3203059724C190612196 @default.
• W3203059724 hasConceptScore W3203059724C203014093 @default.
• W3203059724 hasConceptScore W3203059724C2775867548 @default.
• W3203059724 hasConceptScore W3203059724C2776597655 @default.
• W3203059724 hasConceptScore W3203059724C2778048844 @default.
• W3203059724 hasConceptScore W3203059724C2778371730 @default.
• W3203059724 hasConceptScore W3203059724C2781188995 @default.
• W3203059724 hasConceptScore W3203059724C2781197716 @default.
• W3203059724 hasConceptScore W3203059724C47698986 @default.
• W3203059724 hasConceptScore W3203059724C54355233 @default.
• W3203059724 hasConceptScore W3203059724C60644358 @default.
• W3203059724 hasConceptScore W3203059724C67905577 @default.
• W3203059724 hasConceptScore W3203059724C70721500 @default.
• W3203059724 hasConceptScore W3203059724C86803240 @default.
• W3203059724 hasConceptScore W3203059724C90856448 @default.
• W3203059724 hasFunder F4320306137 @default.

• next