FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3203347720> ?p ?o ?g. }

• W3203347720 endingPage "152" @default.
• W3203347720 startingPage "146" @default.
• W3203347720 abstract "Ischemic stroke (IS) is a common and severe neurological disorder and is associated with high rates of mortality and morbidity. Inflammatory reaction in astrocytes is one of the important pathological factors of stroke. Improved understanding of the underlying molecular mechanisms should aid better treatment of the disease. This study aimed to test our hypothesis that a miR-499a played an important role in the inflammatory response in astrocytes induced by IS targeting phosphatase and tensin homologue deleted on chromosome 10 (PTEN).This study was comprised of two models: oxygen-glucose deprivation (OGD) and reoxygenation model. Quantitative real-time PCR (qRT-PCR) and Western blot were used to examine gene expression levels, and MTT assay analysis were used to examine cell states. The relationships between miR-499a and PTEN were confirmed by luciferase reporter assay.MiR-499a was robustly downregulated with OGD induced injury in astrocytes. Forced transient expression of miR-499a in OGD astrocytes nearly completely reversed the inflammatory response. Knockdown of miR-499a by its specific inhibitor in healthy astrocytes induced the inflammatory response resembling those produced by OGD. On the other hand, PTEN was markedly upregulated in OGD astrocytes, which was reciprocal to the expression of miR-499a. PTEN was experimentally validated as a direct target gene for miR-499a. Overexpression of PTEN was able to induce an inflammatory response of astrocytes. Moreover, PTEN siRNA counteracted the inflammatory response induced by OGD.Taken together, our findings indicate miR-499a as an important factor to prevent inflammatory response and suggest miR-499a as a new molecule for the treatment of IS. The present study also demonstrated the relationship between miR-499a and PTEN, with PTEN as a downstream signaling mediator of miR-499a in the inflammatory response of astrocytes induced by IS." @default.
• W3203347720 created "2021-10-11" @default.
• W3203347720 creator A5013849843 @default.
• W3203347720 creator A5022266933 @default.
• W3203347720 creator A5031372799 @default.
• W3203347720 creator A5035036359 @default.
• W3203347720 creator A5035122818 @default.
• W3203347720 creator A5035198764 @default.
• W3203347720 creator A5057269185 @default.
• W3203347720 creator A5069610659 @default.
• W3203347720 date "2021-09-01" @default.
• W3203347720 modified "2023-10-16" @default.
• W3203347720 title "MiR-499a prevents astrocytes mediated inflammation in ischemic stroke by targeting PTEN" @default.
• W3203347720 cites W1742956183 @default.
• W3203347720 cites W1995569466 @default.
• W3203347720 cites W2001173515 @default.
• W3203347720 cites W2048402284 @default.
• W3203347720 cites W2050640090 @default.
• W3203347720 cites W2071996546 @default.
• W3203347720 cites W2102226828 @default.
• W3203347720 cites W2125386008 @default.
• W3203347720 cites W2153118030 @default.
• W3203347720 cites W2186518561 @default.
• W3203347720 cites W2209965761 @default.
• W3203347720 cites W2239633052 @default.
• W3203347720 cites W2239883203 @default.
• W3203347720 cites W2269676560 @default.
• W3203347720 cites W2296273211 @default.
• W3203347720 cites W2467737812 @default.
• W3203347720 cites W2521218685 @default.
• W3203347720 cites W2556062936 @default.
• W3203347720 cites W2564406489 @default.
• W3203347720 cites W2565789746 @default.
• W3203347720 cites W2582810525 @default.
• W3203347720 cites W265515688 @default.
• W3203347720 cites W2793666111 @default.
• W3203347720 cites W2952900316 @default.
• W3203347720 cites W2975721578 @default.
• W3203347720 cites W2980904583 @default.
• W3203347720 cites W2983011924 @default.
• W3203347720 cites W2989645925 @default.
• W3203347720 cites W2995928844 @default.
• W3203347720 cites W3087432430 @default.
• W3203347720 cites W3095416494 @default.
• W3203347720 cites W3106174716 @default.
• W3203347720 cites W4211070880 @default.
• W3203347720 cites W759793760 @default.
• W3203347720 doi "https://doi.org/10.1016/j.ncrna.2021.09.002" @default.
• W3203347720 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8488463" @default.
• W3203347720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34632168" @default.
• W3203347720 hasPublicationYear "2021" @default.
• W3203347720 type Work @default.
• W3203347720 sameAs 3203347720 @default.
• W3203347720 citedByCount "5" @default.
• W3203347720 countsByYear W32033477202022 @default.
• W3203347720 countsByYear W32033477202023 @default.
• W3203347720 crossrefType "journal-article" @default.
• W3203347720 hasAuthorship W3203347720A5013849843 @default.
• W3203347720 hasAuthorship W3203347720A5022266933 @default.
• W3203347720 hasAuthorship W3203347720A5031372799 @default.
• W3203347720 hasAuthorship W3203347720A5035036359 @default.
• W3203347720 hasAuthorship W3203347720A5035122818 @default.
• W3203347720 hasAuthorship W3203347720A5035198764 @default.
• W3203347720 hasAuthorship W3203347720A5057269185 @default.
• W3203347720 hasAuthorship W3203347720A5069610659 @default.
• W3203347720 hasBestOaLocation W32033477201 @default.
• W3203347720 hasConcept C104317684 @default.
• W3203347720 hasConcept C127561419 @default.
• W3203347720 hasConcept C173396325 @default.
• W3203347720 hasConcept C185592680 @default.
• W3203347720 hasConcept C203014093 @default.
• W3203347720 hasConcept C2776415932 @default.
• W3203347720 hasConcept C2776914184 @default.
• W3203347720 hasConcept C2777609662 @default.
• W3203347720 hasConcept C2779549131 @default.
• W3203347720 hasConcept C502942594 @default.
• W3203347720 hasConcept C55493867 @default.
• W3203347720 hasConcept C62478195 @default.
• W3203347720 hasConcept C71924100 @default.
• W3203347720 hasConcept C86554907 @default.
• W3203347720 hasConceptScore W3203347720C104317684 @default.
• W3203347720 hasConceptScore W3203347720C127561419 @default.
• W3203347720 hasConceptScore W3203347720C173396325 @default.
• W3203347720 hasConceptScore W3203347720C185592680 @default.
• W3203347720 hasConceptScore W3203347720C203014093 @default.
• W3203347720 hasConceptScore W3203347720C2776415932 @default.
• W3203347720 hasConceptScore W3203347720C2776914184 @default.
• W3203347720 hasConceptScore W3203347720C2777609662 @default.
• W3203347720 hasConceptScore W3203347720C2779549131 @default.
• W3203347720 hasConceptScore W3203347720C502942594 @default.
• W3203347720 hasConceptScore W3203347720C55493867 @default.
• W3203347720 hasConceptScore W3203347720C62478195 @default.
• W3203347720 hasConceptScore W3203347720C71924100 @default.
• W3203347720 hasConceptScore W3203347720C86554907 @default.
• W3203347720 hasIssue "3" @default.
• W3203347720 hasLocation W32033477201 @default.
• W3203347720 hasLocation W32033477202 @default.
• W3203347720 hasLocation W32033477203 @default.

• next