FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3203669241> ?p ?o ?g. }

• W3203669241 endingPage "155" @default.
• W3203669241 startingPage "144" @default.
• W3203669241 abstract "The reprogramming of cellular metabolism is a hallmark of cancer. The ability to noninvasively assay glucose and lactate concentrations in cancer cells would improve our understanding of the dynamic changes in metabolic activity accompanying tumor initiation, progression, and response to therapy. Unfortunately, common approaches for measuring these nutrient levels are invasive or interrupt cell growth. This study transfected FRET reporters quantifying glucose and lactate concentration into breast cancer cell lines to study nutrient dynamics and response to therapy.Two FRET reporters, one assaying glucose concentration and one assaying lactate concentration, were stably transfected into the MDA-MB-231 breast cancer cell line. Correlation between FRET measurements and ligand concentration were measured using a confocal microscope and a cell imaging plate reader. Longitudinal changes in glucose and lactate concentration were measured in response to treatment with CoCl2, cytochalasin B, and phloretin which, respectively, induce hypoxia, block glucose uptake, and block glucose and lactate transport.The FRET ratio from the glucose and lactate reporters increased with increasing concentration of the corresponding ligand (p < 0.005 and p < 0.05, respectively). The FRET ratio from both reporters was found to decrease over time for high initial concentrations of the ligand (p < 0.01). Significant differences in the FRET ratio corresponding to metabolic inhibition were found when cells were treated with glucose/lactate transporter inhibitors.FRET reporters can track intracellular glucose and lactate dynamics in cancer cells, providing insight into tumor metabolism and response to therapy over time." @default.
• W3203669241 created "2021-10-11" @default.
• W3203669241 creator A5014942870 @default.
• W3203669241 creator A5016057283 @default.
• W3203669241 creator A5027619317 @default.
• W3203669241 creator A5034344273 @default.
• W3203669241 creator A5054237973 @default.
• W3203669241 creator A5063526365 @default.
• W3203669241 creator A5065711135 @default.
• W3203669241 creator A5081951602 @default.
• W3203669241 date "2021-10-05" @default.
• W3203669241 modified "2023-10-16" @default.
• W3203669241 title "Longitudinal FRET Imaging of Glucose and Lactate Dynamics and Response to Therapy in Breast Cancer Cells" @default.
• W3203669241 cites W1455672147 @default.
• W3203669241 cites W1970558444 @default.
• W3203669241 cites W1970996670 @default.
• W3203669241 cites W1971859682 @default.
• W3203669241 cites W1978080197 @default.
• W3203669241 cites W2005165301 @default.
• W3203669241 cites W2028897202 @default.
• W3203669241 cites W2029436635 @default.
• W3203669241 cites W2031299955 @default.
• W3203669241 cites W2045651327 @default.
• W3203669241 cites W2047195788 @default.
• W3203669241 cites W2055755990 @default.
• W3203669241 cites W2064380858 @default.
• W3203669241 cites W2072451938 @default.
• W3203669241 cites W2097259163 @default.
• W3203669241 cites W2110222089 @default.
• W3203669241 cites W2117305340 @default.
• W3203669241 cites W2117692326 @default.
• W3203669241 cites W2119255682 @default.
• W3203669241 cites W2122372985 @default.
• W3203669241 cites W2122995470 @default.
• W3203669241 cites W2123308800 @default.
• W3203669241 cites W2149902856 @default.
• W3203669241 cites W2158453778 @default.
• W3203669241 cites W2202641067 @default.
• W3203669241 cites W2224116510 @default.
• W3203669241 cites W2229267816 @default.
• W3203669241 cites W2318944285 @default.
• W3203669241 cites W2419165202 @default.
• W3203669241 cites W2592319176 @default.
• W3203669241 cites W2607436811 @default.
• W3203669241 cites W2611496024 @default.
• W3203669241 cites W2739924689 @default.
• W3203669241 cites W2755677304 @default.
• W3203669241 cites W2802991505 @default.
• W3203669241 cites W2808457964 @default.
• W3203669241 cites W2887716539 @default.
• W3203669241 cites W2890250729 @default.
• W3203669241 cites W2984651242 @default.
• W3203669241 cites W2984975897 @default.
• W3203669241 cites W3048422988 @default.
• W3203669241 cites W3107871538 @default.
• W3203669241 cites W3126154645 @default.
• W3203669241 cites W3131393213 @default.
• W3203669241 cites W4230489839 @default.
• W3203669241 cites W4237750710 @default.
• W3203669241 doi "https://doi.org/10.1007/s11307-021-01639-4" @default.
• W3203669241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34611767" @default.
• W3203669241 hasPublicationYear "2021" @default.
• W3203669241 type Work @default.
• W3203669241 sameAs 3203669241 @default.
• W3203669241 citedByCount "3" @default.
• W3203669241 countsByYear W32036692412022 @default.
• W3203669241 countsByYear W32036692412023 @default.
• W3203669241 crossrefType "journal-article" @default.
• W3203669241 hasAuthorship W3203669241A5014942870 @default.
• W3203669241 hasAuthorship W3203669241A5016057283 @default.
• W3203669241 hasAuthorship W3203669241A5027619317 @default.
• W3203669241 hasAuthorship W3203669241A5034344273 @default.
• W3203669241 hasAuthorship W3203669241A5054237973 @default.
• W3203669241 hasAuthorship W3203669241A5063526365 @default.
• W3203669241 hasAuthorship W3203669241A5065711135 @default.
• W3203669241 hasAuthorship W3203669241A5081951602 @default.
• W3203669241 hasBestOaLocation W32036692412 @default.
• W3203669241 hasConcept C121332964 @default.
• W3203669241 hasConcept C121608353 @default.
• W3203669241 hasConcept C12554922 @default.
• W3203669241 hasConcept C126322002 @default.
• W3203669241 hasConcept C134018914 @default.
• W3203669241 hasConcept C161573976 @default.
• W3203669241 hasConcept C185592680 @default.
• W3203669241 hasConcept C2777866211 @default.
• W3203669241 hasConcept C2779306644 @default.
• W3203669241 hasConcept C55493867 @default.
• W3203669241 hasConcept C62520636 @default.
• W3203669241 hasConcept C71924100 @default.
• W3203669241 hasConcept C86803240 @default.
• W3203669241 hasConcept C91881484 @default.
• W3203669241 hasConcept C96232424 @default.
• W3203669241 hasConcept C96305047 @default.
• W3203669241 hasConceptScore W3203669241C121332964 @default.
• W3203669241 hasConceptScore W3203669241C121608353 @default.
• W3203669241 hasConceptScore W3203669241C12554922 @default.
• W3203669241 hasConceptScore W3203669241C126322002 @default.
• W3203669241 hasConceptScore W3203669241C134018914 @default.

• next