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• W3203736701 abstract "Background Preterm infants have high rates of morbidity, especially from late-onset sepsis and necrotising enterocolitis. Lactoferrin is an anti-infective milk protein that may act through effects on gut bacteria, metabolites and epithelial cell function. The impact of supplemental lactoferrin in reducing late-onset sepsis was explored in the Enteral LactoFerrin In Neonates (ELFIN) trial. Objectives The Mechanisms Affecting the Gut of Preterm Infants in Enteral feeding (MAGPIE) study was nested within the ELFIN trial and aimed to determine the impact of lactoferrin on gut microbiota and bacterial function, and changes preceding disease onset. We aimed to explore impacts on the stool bacteria and faecal/urinary metabolome using gas and liquid chromatography–mass spectrometry, and explore immunohistological pathways in resected tissue. Methods Preterm infants from 12 NHS hospitals were enrolled in the study, and daily stool and urine samples were collected. Local sample collection data were combined with ELFIN trial data from the National Perinatal Epidemiology Unit, Oxford. The longitudinal impact of lactoferrin in healthy infants was determined, and samples that were collected before disease onset were matched with samples from healthy control infants. Established, quality-controlled 16S ribonucleic acid, gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry analyses were conducted. Validated databases and standardised workflows were used to identify bacteria and metabolites. Tissue samples from infants undergoing surgery and matched controls were analysed. Results We recruited 479 preterm infants (mean gestation of 28.4 ± 2.3 weeks) and collected > 33,000 usable samples from 467 infants. 16S ribonucleic acid bacterial analysis was conducted on samples from 201 infants, of whom 20 had necrotising enterocolitis and 51 had late-onset sepsis, along with samples from healthy matched controls to explore longitudinal changes. The greatest change in relative bacterial abundance over time was observed in Staphylococcus , which decreased from 42% at aged 7–9 days to only 2% at aged 30–60 days ( p < 0.001). Small but significant differences in community composition were observed between samples in each ELFIN trial group ( R 2 = 0.005; p = 0.04). Staphylococcus ( p < 0.01), Haemophilus ( p < 0.01) and Lactobacillus ( p = 0.01) showed greater mean relative abundance in the placebo group than in the lactoferrin group. Gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry analyses showed that lactoferrin had limited impact on the metabolome. Liquid chromatography–mass spectrometry showed significant metabolite differences between necrotising enterocolitis or late-onset sepsis infants and healthy controls. The resected gut tissue analysis revealed 82 differentially expressed genes between healthy and necrotic tissue. Limitations Although we recruited a large number of infants, collecting daily samples from every infant is challenging, especially in the few days immediately preceding disease onset. Conclusion We conducted a large mechanistic study across multiple hospital sites and showed that, although lactoferrin significantly decreased the level of Staphylococcus and other key pathogens, the impact was smaller than those of other clinical variables. Immunohistochemistry identified multiple inflammatory pathways leading to necrotising enterocolitis and showed that the use of NHS pathology archive tissue is feasible in the context of a randomised controlled trial. Future work We observed significant changes in the stool and urinary metabolome in cases preceding late-onset sepsis or necrotising enterocolitis, which provide metabolic targets for a future mechanistic and biomarker study. Trial registration Current Controlled Trials ISRCTN12554594. Funding This project was funded by the Efficacy and Mechanism Evaluation (EME) programme, a Medical Research Council (MRC) and National Institute for Health Research (NIHR) partnership. This will be published in full in Efficacy and Mechanism Evaluation ; Vol. 8, No. 14. See the NIHR Journals Library website for further project information." @default.
• W3203736701 created "2021-10-11" @default.
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• W3203736701 date "2021-09-01" @default.
• W3203736701 modified "2023-09-28" @default.
• W3203736701 title "Lactoferrin impact on gut microbiota in preterm infants with late-onset sepsis or necrotising enterocolitis: the MAGPIE mechanisms of action study" @default.
• W3203736701 cites W1480377400 @default.
• W3203736701 cites W1512811072 @default.
• W3203736701 cites W1611854178 @default.
• W3203736701 cites W1811260695 @default.
• W3203736701 cites W1951724000 @default.
• W3203736701 cites W1964010911 @default.
• W3203736701 cites W1965262430 @default.
• W3203736701 cites W1976367503 @default.
• W3203736701 cites W1999906527 @default.
• W3203736701 cites W2012134097 @default.
• W3203736701 cites W2014346243 @default.
• W3203736701 cites W2020731315 @default.
• W3203736701 cites W2027338113 @default.
• W3203736701 cites W2032544480 @default.
• W3203736701 cites W2033115012 @default.
• W3203736701 cites W2034285706 @default.
• W3203736701 cites W2056279562 @default.
• W3203736701 cites W2057100837 @default.
• W3203736701 cites W2062901773 @default.
• W3203736701 cites W2073939393 @default.
• W3203736701 cites W2076461297 @default.
• W3203736701 cites W2077304943 @default.
• W3203736701 cites W2078931266 @default.
• W3203736701 cites W2080283023 @default.
• W3203736701 cites W208036677 @default.
• W3203736701 cites W2081164775 @default.
• W3203736701 cites W2081465448 @default.
• W3203736701 cites W2083637865 @default.
• W3203736701 cites W2087195457 @default.
• W3203736701 cites W2093701408 @default.
• W3203736701 cites W2100316629 @default.
• W3203736701 cites W2102756828 @default.
• W3203736701 cites W2105024467 @default.
• W3203736701 cites W2108718991 @default.
• W3203736701 cites W2109342699 @default.
• W3203736701 cites W2117457769 @default.
• W3203736701 cites W2118117266 @default.
• W3203736701 cites W2121851775 @default.
• W3203736701 cites W2121949313 @default.
• W3203736701 cites W2124087884 @default.
• W3203736701 cites W2125644218 @default.
• W3203736701 cites W2128991285 @default.
• W3203736701 cites W2134591191 @default.
• W3203736701 cites W2139711652 @default.
• W3203736701 cites W2160948622 @default.
• W3203736701 cites W2166212175 @default.
• W3203736701 cites W2167176937 @default.
• W3203736701 cites W2168150136 @default.
• W3203736701 cites W2174358501 @default.
• W3203736701 cites W2182173893 @default.
• W3203736701 cites W2254549833 @default.
• W3203736701 cites W2269609486 @default.
• W3203736701 cites W2295620997 @default.
• W3203736701 cites W2297973418 @default.
• W3203736701 cites W2326630044 @default.
• W3203736701 cites W2395592142 @default.
• W3203736701 cites W2406474639 @default.
• W3203736701 cites W2478228532 @default.
• W3203736701 cites W2551850626 @default.
• W3203736701 cites W2561338758 @default.
• W3203736701 cites W2574079157 @default.
• W3203736701 cites W2581560082 @default.
• W3203736701 cites W2587011319 @default.
• W3203736701 cites W2592537218 @default.
• W3203736701 cites W2605603608 @default.
• W3203736701 cites W2612161536 @default.
• W3203736701 cites W2612332169 @default.
• W3203736701 cites W2616217075 @default.
• W3203736701 cites W2623350814 @default.
• W3203736701 cites W2731811823 @default.
• W3203736701 cites W2734475592 @default.
• W3203736701 cites W2740017040 @default.
• W3203736701 cites W2751401859 @default.
• W3203736701 cites W2760923115 @default.
• W3203736701 cites W2762056035 @default.
• W3203736701 cites W2775023903 @default.

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