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- W3203762317 endingPage "1495.e12" @default.
- W3203762317 startingPage "1482" @default.
- W3203762317 abstract "CRISPR-Cas systems provide immunity to bacteria by programing Cas nucleases with RNA guides that recognize and cleave infecting viral genomes. Bacteria and their viruses each encode recombination systems that could repair the cleaved viral DNA. However, it is unknown whether and how these systems can affect CRISPR immunity. Bacteriophage λ uses the Red system (gam-exo-bet) to promote recombination between related phages. Here, we show that λ Red also mediates evasion of CRISPR-Cas targeting. Gam inhibits the host E. coli RecBCD recombination system, allowing recombination and repair of the cleaved DNA by phage Exo-Beta, which promotes the generation of mutations within the CRISPR target sequence. Red recombination is strikingly more efficient than the host's RecBCD-RecA in the production of large numbers of phages that escape CRISPR targeting. These results reveal a role for Red-like systems in the protection of bacteriophages against sequence-specific nucleases, which may facilitate their spread across viral genomes." @default.
- W3203762317 created "2021-10-11" @default.
- W3203762317 creator A5024206759 @default.
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- W3203762317 creator A5064100051 @default.
- W3203762317 creator A5064556913 @default.
- W3203762317 creator A5087893982 @default.
- W3203762317 date "2021-10-01" @default.
- W3203762317 modified "2023-09-29" @default.
- W3203762317 title "Viral recombination systems limit CRISPR-Cas targeting through the generation of escape mutations" @default.
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- W3203762317 doi "https://doi.org/10.1016/j.chom.2021.09.001" @default.
- W3203762317 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8516739" @default.
- W3203762317 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34582782" @default.
- W3203762317 hasPublicationYear "2021" @default.
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