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• W3203765354 abstract "Preanalytical errors causing specimen contamination with zinc (Zn) are disastrous for routine medical diagnostics or scientific studies. The aim of the study presented here is to simulate contamination possibilities when using single-use gloves. The ability to release Zn into the specimen was tested using nitril (A), vinyl (B) and latex (C) gloves with 15 (14) replications. In our first approach, a 1 × 1 cm piece of the glove's fingertip was incubated for 10 min with serum. Our second approach imitated a very short contact of serum to the glove's material by letting serum run over the glove from a pipette for 3 cm distance into a tube. The effect of gloves' contact to liver tissue was examined using glove C only: a block of liver tissue was touched once at one side producing an experimental fingerprint. Zn was analyzed in serum and liver wet weight (ww) using ICP-MS; the basal serum/liver Zn concentration was set as zero for calculation. The calculated addition of Zn is given as median (p25 - p75). The first approach led to distinct contamination with Zn (in μg/L) being evident from all three types of gloves, but depended markedly from the type of material: A: 176.5 (129.7-204); B: 975.1 (663.6-1164.3); C: 2112 (1685-2516). Imitating a very short contact of serum to the glove's surface resulted in an additional Zn concentration of 105.7 (70.4-168.8), 56.2 (-13.5-121.4) and 955.7 (746-1159) μg/L using gloves A, B and C, respectively. A single fingerprint on liver tissue using glove C resulted an addition of 3995 (861-6435) μg Zn/kg liver ww. The data underline that the dimension of preanalytical contamination of blood and tissue samples for Zn analysis via single-use gloves is relevant for routine diagnostics and scientific studies. Critical steps and possibilities to minimize these effects should be considered seriously for specimen handling in routine laboratory diagnostics as well as in scientific studies to avoid preanalytical errors and, finally, misinterpretation of the data." @default.
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• W3203765354 date "2021-12-01" @default.
• W3203765354 modified "2023-09-27" @default.
• W3203765354 title "Preanalytical errors on zinc concentrations caused by single-use gloves" @default.
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• W3203765354 doi "https://doi.org/10.1016/j.jtemb.2021.126871" @default.
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