FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204019051> ?p ?o ?g. }

• W3204019051 abstract "ABSTRACT Human adenovirus serotype 26 (Ad26) is used as a gene-based vaccine against SARS-CoV-2 and HIV-1. Yet, its primary receptor portfolio remains controversial, potentially including sialic acid, CAR, integrins, and CD46. We and others have shown that Ad26 can use CD46, but these observations were questioned by the inability to co-crystallize Ad26 fiber with CD46. Recent work demonstrated that Ad26 binds CD46 with its hexon protein rather than its fiber. We examined the functional consequences of Ad26 for infection in vitro and in vivo . Ectopic expression of human CD46 on Chinese hamster ovary cells increased Ad26 infection significantly. Deletion of the complement control protein domains CCP1 or CCP2 or the serine-threonine-proline (STP) region of CD46 reduced infection. Comparing wild type and sialic acid-deficient CHO cells, we show that the usage of CD46 is independent of its sialylation status. Ad26 transduction was increased in CD46 transgenic mice after intramuscular (IM) injection, but not after intranasal (IN) administration. Ad26 transduction was 10-fold lower than Ad5 after intratumoral (IT) injection of CD46-expressing tumors. Ad26 transduction of liver was 1000-fold lower than Ad5 after intravenous (IV) injection. These data demonstrate the use of CD46 by Ad26 under certain situations, but also show that the receptor has little consequence by other routes of administration. Finally, IV injection of high doses of Ad26 into CD46 mice induced release of liver enzymes in the bloodstream and reduced white blood cell counts, but did not induce thrombocytopenia. This suggests that Ad26 virions do not induce direct clotting side effects seen during COVID-19 vaccination with this serotype of adenovirus. IMPORTANCE Human species D Ad26 is being pursued as a low seroprevalence vector for oncolytic virotherapy and gene-based vaccination against HIV-1 and SARS-CoV-2. However, there is debate in the literature about its tropism and receptor utilization, which directly influence its efficiency for certain applications. This work was aimed at determining which receptor(s) this virus uses for infection, and its role in virus biology, vaccine efficacy, and importantly, in vaccine safety." @default.
• W3204019051 created "2021-10-11" @default.
• W3204019051 creator A5005265839 @default.
• W3204019051 creator A5007439954 @default.
• W3204019051 creator A5008491017 @default.
• W3204019051 creator A5013958915 @default.
• W3204019051 creator A5021951430 @default.
• W3204019051 creator A5026834279 @default.
• W3204019051 creator A5029265500 @default.
• W3204019051 creator A5065993014 @default.
• W3204019051 creator A5073092010 @default.
• W3204019051 creator A5074296211 @default.
• W3204019051 creator A5081608724 @default.
• W3204019051 creator A5082437028 @default.
• W3204019051 creator A5084158740 @default.
• W3204019051 creator A5090830842 @default.
• W3204019051 date "2021-10-01" @default.
• W3204019051 modified "2023-09-27" @default.
• W3204019051 title "<i>Ex Vivo</i>and<i>In Vivo</i>CD46 Receptor Utilization by Species D Human Adenovirus Serotype 26 (HAdV26)" @default.
• W3204019051 cites W1528082581 @default.
• W3204019051 cites W1581511516 @default.
• W3204019051 cites W1607950606 @default.
• W3204019051 cites W1656961899 @default.
• W3204019051 cites W1930355415 @default.
• W3204019051 cites W1965869797 @default.
• W3204019051 cites W1968487372 @default.
• W3204019051 cites W1986864754 @default.
• W3204019051 cites W1992762917 @default.
• W3204019051 cites W1998150511 @default.
• W3204019051 cites W1998397400 @default.
• W3204019051 cites W2007270424 @default.
• W3204019051 cites W2018067024 @default.
• W3204019051 cites W2027145049 @default.
• W3204019051 cites W2027980197 @default.
• W3204019051 cites W2028068426 @default.
• W3204019051 cites W2028633221 @default.
• W3204019051 cites W2029195580 @default.
• W3204019051 cites W2040274499 @default.
• W3204019051 cites W2040941118 @default.
• W3204019051 cites W2057034062 @default.
• W3204019051 cites W2057359971 @default.
• W3204019051 cites W2057834288 @default.
• W3204019051 cites W2058278883 @default.
• W3204019051 cites W2067110265 @default.
• W3204019051 cites W2073755366 @default.
• W3204019051 cites W2076378943 @default.
• W3204019051 cites W2081966992 @default.
• W3204019051 cites W2083415337 @default.
• W3204019051 cites W2084836020 @default.
• W3204019051 cites W2085096592 @default.
• W3204019051 cites W2091945720 @default.
• W3204019051 cites W2094030754 @default.
• W3204019051 cites W2105375220 @default.
• W3204019051 cites W2109699279 @default.
• W3204019051 cites W2116071714 @default.
• W3204019051 cites W2119545820 @default.
• W3204019051 cites W2129380034 @default.
• W3204019051 cites W2131315410 @default.
• W3204019051 cites W2134119060 @default.
• W3204019051 cites W2144957027 @default.
• W3204019051 cites W2151130810 @default.
• W3204019051 cites W2152020672 @default.
• W3204019051 cites W2153792353 @default.
• W3204019051 cites W2161622051 @default.
• W3204019051 cites W2169962588 @default.
• W3204019051 cites W2277215203 @default.
• W3204019051 cites W2321723369 @default.
• W3204019051 cites W2415663312 @default.
• W3204019051 cites W2569074527 @default.
• W3204019051 cites W2612394209 @default.
• W3204019051 cites W2889535280 @default.
• W3204019051 cites W2897732259 @default.
• W3204019051 cites W2952343158 @default.
• W3204019051 cites W2972098631 @default.
• W3204019051 cites W3045734160 @default.
• W3204019051 cites W3083750089 @default.
• W3204019051 cites W3115163609 @default.
• W3204019051 cites W3140226555 @default.
• W3204019051 cites W3152794373 @default.
• W3204019051 cites W3161418487 @default.
• W3204019051 doi "https://doi.org/10.1101/2021.09.28.462271" @default.
• W3204019051 hasPublicationYear "2021" @default.
• W3204019051 type Work @default.
• W3204019051 sameAs 3204019051 @default.
• W3204019051 citedByCount "1" @default.
• W3204019051 countsByYear W32040190512022 @default.
• W3204019051 crossrefType "posted-content" @default.
• W3204019051 hasAuthorship W3204019051A5005265839 @default.
• W3204019051 hasAuthorship W3204019051A5007439954 @default.
• W3204019051 hasAuthorship W3204019051A5008491017 @default.
• W3204019051 hasAuthorship W3204019051A5013958915 @default.
• W3204019051 hasAuthorship W3204019051A5021951430 @default.
• W3204019051 hasAuthorship W3204019051A5026834279 @default.
• W3204019051 hasAuthorship W3204019051A5029265500 @default.
• W3204019051 hasAuthorship W3204019051A5065993014 @default.
• W3204019051 hasAuthorship W3204019051A5073092010 @default.
• W3204019051 hasAuthorship W3204019051A5074296211 @default.
• W3204019051 hasAuthorship W3204019051A5081608724 @default.
• W3204019051 hasAuthorship W3204019051A5082437028 @default.
• W3204019051 hasAuthorship W3204019051A5084158740 @default.

• next