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- W3204096580 abstract "Embryonic stem cells (ESCs) are derived from the inner cell mass of the preimplantation blastocyst and can be maintained indefinitely in vitro without losing their properties. Given their self-renewal and pluripotency, ESCs not only represent a key tool to study early embryonic development in a dish, but also an unlimited source of material for tissue replacement in regenerative medicine. Loss-of-function assays using RNA interference are a powerful tool to understand the roles of specific genes and are facilitated by lentiviral-mediated delivery of vector-encoded shRNAs which allows long-term silencing of single or multiple genes. Here, we describe the steps for rapid and cost-effective production and testing of lentiviral particles with vector-encoded shRNAs for gene silencing in ESCs. This protocol can be easily adapted for loss-of-function assays in other pluripotent cells or culture conditions of interest." @default.
- W3204096580 created "2021-10-11" @default.
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- W3204096580 date "2021-01-01" @default.
- W3204096580 modified "2023-09-25" @default.
- W3204096580 title "A Simple, Rapid, and Cost-Effective Method for Loss-of-Function Assays in Pluripotent Cells" @default.
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- W3204096580 doi "https://doi.org/10.1007/7651_2021_434" @default.
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