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- W3204102748 abstract "The refractory organics released from waste activated sludge (WAS) are unwanted produced in thermal-alkaline pretreatment, which are not well documented. In this study, we refer to them as melanoidins products (MPs) with characteristics of high molecular weight and inhibition to microbes. The results showed that these MPs from thermal-alkaline (80 °C and pH 10) pretreatment of WAS were identified with a broad molecular weight (>1000 Da). Dark-colored MPs were further verified from glucose and tryptophan as the model components, with values of UV280 and UV420 increasing. The produced MPs with a molecular weight of 1220, 6835, and even 21,200,000 Da were confirmed by SEC-HPLC. Unexpectedly, MPs were found to be electroactive with higher redox peak values than that of humic acids, which were almost not degraded by anaerobes as revealed by SEC-HPLC and 3D-EEM spectra. For the first time, the results demonstrated that MPs delayed volatile fatty acids production and reduced the methane yield (22-26% lower), which was likely attributed to the toxicity and/or electrons competition with anaerobes such as Methanosaeta. Thus, it is clear that MPs negatively impact anaerobic digestion after thermal-alkaline pretreatment, which shall be re-evaluated to minimize MPs when producing biochemicals from WAS." @default.
- W3204102748 created "2021-10-11" @default.
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- W3204102748 date "2022-02-01" @default.
- W3204102748 modified "2023-10-14" @default.
- W3204102748 title "Elucidating the production and inhibition of melanoidins products on anaerobic digestion after thermal-alkaline pretreatment" @default.
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- W3204102748 doi "https://doi.org/10.1016/j.jhazmat.2021.127377" @default.
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