Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204114465> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W3204114465 endingPage "24" @default.
- W3204114465 startingPage "19" @default.
- W3204114465 abstract "The increase in the frequency of drug resistance in bacterial infections has led to the research of new antibacterial agents targeting new mechanisms. Many of the functions of NAD+-dependent DNA ligase have made it a remarkable target for antibacterial drug discovery. Escherichia coli (E. coli) NAD+-dependent DNA ligase is presented as a potential target due to its unique substrate specificity compared to the ATP-dependent human DNA ligase. In this study, it was aimed to produce and purify the E. coli NAD + dependent DNA ligase enzyme, which is frequently used in antibacterial drug discovery. The E. coli DNA ligase gene sequence was cloned into pTOLT vector system. E. coli DNA ligase enzyme was purified after the production in E. coli BL21 (DE3) pLysE cells. It was clearly demonstrated by the activity test that the DNA ligase enzyme produced in this study can ligate the DNA fragments. As a result, it was revealed that the effect of candidate inhibitors can be studied simply on the enzyme." @default.
- W3204114465 created "2021-10-11" @default.
- W3204114465 creator A5015877882 @default.
- W3204114465 creator A5018487339 @default.
- W3204114465 creator A5038189677 @default.
- W3204114465 date "2022-02-28" @default.
- W3204114465 modified "2023-09-28" @default.
- W3204114465 title "Antibakteriyel İlaç Keşfi İçin Bir Hedef Olarak E. Coli NAD+-Bağımlı DNA Ligazın Rekombinant Üretimi" @default.
- W3204114465 cites W1583908667 @default.
- W3204114465 cites W1597820140 @default.
- W3204114465 cites W1886571240 @default.
- W3204114465 cites W1967354453 @default.
- W3204114465 cites W1970391898 @default.
- W3204114465 cites W1976548111 @default.
- W3204114465 cites W1977024992 @default.
- W3204114465 cites W1977971905 @default.
- W3204114465 cites W1978978605 @default.
- W3204114465 cites W1998834249 @default.
- W3204114465 cites W2001000225 @default.
- W3204114465 cites W2006327015 @default.
- W3204114465 cites W2010259190 @default.
- W3204114465 cites W2033302551 @default.
- W3204114465 cites W2037240454 @default.
- W3204114465 cites W2043164524 @default.
- W3204114465 cites W2067297732 @default.
- W3204114465 cites W2071542814 @default.
- W3204114465 cites W2090925521 @default.
- W3204114465 cites W2108092697 @default.
- W3204114465 cites W2115121941 @default.
- W3204114465 cites W2118028651 @default.
- W3204114465 cites W2124182498 @default.
- W3204114465 cites W2125735909 @default.
- W3204114465 cites W2169373287 @default.
- W3204114465 cites W2170809234 @default.
- W3204114465 cites W3147510950 @default.
- W3204114465 doi "https://doi.org/10.18016/ksutarimdoga.vi.884279" @default.
- W3204114465 hasPublicationYear "2022" @default.
- W3204114465 type Work @default.
- W3204114465 sameAs 3204114465 @default.
- W3204114465 citedByCount "0" @default.
- W3204114465 crossrefType "journal-article" @default.
- W3204114465 hasAuthorship W3204114465A5015877882 @default.
- W3204114465 hasAuthorship W3204114465A5018487339 @default.
- W3204114465 hasAuthorship W3204114465A5038189677 @default.
- W3204114465 hasBestOaLocation W32041144651 @default.
- W3204114465 hasConcept C104317684 @default.
- W3204114465 hasConcept C153911025 @default.
- W3204114465 hasConcept C175114707 @default.
- W3204114465 hasConcept C181199279 @default.
- W3204114465 hasConcept C187882448 @default.
- W3204114465 hasConcept C19924922 @default.
- W3204114465 hasConcept C203284983 @default.
- W3204114465 hasConcept C2908738455 @default.
- W3204114465 hasConcept C47558249 @default.
- W3204114465 hasConcept C49105822 @default.
- W3204114465 hasConcept C547475151 @default.
- W3204114465 hasConcept C552990157 @default.
- W3204114465 hasConcept C55493867 @default.
- W3204114465 hasConcept C75520062 @default.
- W3204114465 hasConcept C86803240 @default.
- W3204114465 hasConcept C90583042 @default.
- W3204114465 hasConceptScore W3204114465C104317684 @default.
- W3204114465 hasConceptScore W3204114465C153911025 @default.
- W3204114465 hasConceptScore W3204114465C175114707 @default.
- W3204114465 hasConceptScore W3204114465C181199279 @default.
- W3204114465 hasConceptScore W3204114465C187882448 @default.
- W3204114465 hasConceptScore W3204114465C19924922 @default.
- W3204114465 hasConceptScore W3204114465C203284983 @default.
- W3204114465 hasConceptScore W3204114465C2908738455 @default.
- W3204114465 hasConceptScore W3204114465C47558249 @default.
- W3204114465 hasConceptScore W3204114465C49105822 @default.
- W3204114465 hasConceptScore W3204114465C547475151 @default.
- W3204114465 hasConceptScore W3204114465C552990157 @default.
- W3204114465 hasConceptScore W3204114465C55493867 @default.
- W3204114465 hasConceptScore W3204114465C75520062 @default.
- W3204114465 hasConceptScore W3204114465C86803240 @default.
- W3204114465 hasConceptScore W3204114465C90583042 @default.
- W3204114465 hasIssue "1" @default.
- W3204114465 hasLocation W32041144651 @default.
- W3204114465 hasLocation W32041144652 @default.
- W3204114465 hasOpenAccess W3204114465 @default.
- W3204114465 hasPrimaryLocation W32041144651 @default.
- W3204114465 hasRelatedWork W1553799050 @default.
- W3204114465 hasRelatedWork W1587606408 @default.
- W3204114465 hasRelatedWork W1992278173 @default.
- W3204114465 hasRelatedWork W2013087839 @default.
- W3204114465 hasRelatedWork W2059461836 @default.
- W3204114465 hasRelatedWork W2073788366 @default.
- W3204114465 hasRelatedWork W2162737386 @default.
- W3204114465 hasRelatedWork W3107404870 @default.
- W3204114465 hasRelatedWork W3173234305 @default.
- W3204114465 hasRelatedWork W3204114465 @default.
- W3204114465 hasVolume "25" @default.
- W3204114465 isParatext "false" @default.
- W3204114465 isRetracted "false" @default.
- W3204114465 magId "3204114465" @default.
- W3204114465 workType "article" @default.