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• W3204126043 endingPage "132401" @default.
• W3204126043 startingPage "132401" @default.
• W3204126043 abstract "The progressive increase of slaughterhouse waste production requires actions for both addressing an environmental issue and creating additional value within a biorefinery concept. In this regard, some of these animal by-products exhibit a significant content of fatty acids that could be efficiently converted into bioplastics such as polyhydroxyalkanoates (PHAs) by adequately performing substrate screening with producing bacterial strains and applying affordable pretreatments. One of the main challenges also relies on the difficulty to emulsify these fat-rich substrates within culture broth and make the fatty acids accessible for the producing bacteria. In this work, the potential of two fat-rich animal by-products, grease trap waste (GTW) and tallow-based jelly (TBJ), as inexpensive carbon sources for microbial growth and PHA production was evaluated for the first time. Upon substrate screening, using different pseudomonadal strains (P. resinovorans, P. putida GPo1, P. putida KT2440) and pretreatment conditions (autoclave-based, thermally-treated or saponified substrates), the highest growth and mcl-PHA production performance was obtained for P. resinovorans, thus producing up to 47% w/w mcl-PHA simply using hygienized GTW. The novel bioprocess described in this study was successfully scaled up to 5 and 15 L, resulting in CDW concentrations of 5.9-12.8 g L-1, mcl-PHA contents of 33-62% w/w and PHA yields of 0.1-0.4 gPHA g-1fatty acids, greatly depending on the substrate dosing strategy used and depending on culture conditions. Moreover, process robustness was confirmed along Test Series by the roughly stable monomeric composition of the biopolymer produced, mainly formed by 3-hydroxyoctanoate and 3-hydroxydecanoate. The research here conducted is crucial for the cost-effectiveness of mcl-PHA production along this new slaughterhouse waste-based biorefinery concept." @default.
• W3204126043 created "2021-10-11" @default.
• W3204126043 creator A5038346786 @default.
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• W3204126043 date "2022-01-01" @default.
• W3204126043 modified "2023-10-18" @default.
• W3204126043 title "Exploring the potential of slaughterhouse waste valorization: Development and scale-up of a new bioprocess for medium-chain length polyhydroxyalkanoates production" @default.
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• W3204126043 doi "https://doi.org/10.1016/j.chemosphere.2021.132401" @default.
• W3204126043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34600930" @default.
• W3204126043 hasPublicationYear "2022" @default.
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