Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204151063> ?p ?o ?g. }
- W3204151063 abstract "Abstract Despite four decades of research to support the association between DNA methylation and gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that experimental confounds preclude resolution of this question with existing strategies, including recently developed CRISPR/dCas9 and TET-based epigenetic editors. Instead, we demonstrate a highly effective method using only nuclease-dead Cas9 and guide RNA to physically block DNA methylation at specific targets in the absence of a confounding flexibly-tethered enzyme, thereby enabling the examination of the role of DNA demethylation per se in living cells, with no evidence of off-target activity. Using this method, we probe a small number of inducible promoters and find the effect of DNA demethylation to be small, while demethylation of CpG-rich FMR1 produces larger changes in gene expression. This method could be used to reveal the extent and nature of the contribution of DNA methylation to gene regulation." @default.
- W3204151063 created "2021-10-11" @default.
- W3204151063 creator A5007458839 @default.
- W3204151063 creator A5011707289 @default.
- W3204151063 date "2021-09-29" @default.
- W3204151063 modified "2023-10-04" @default.
- W3204151063 title "Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9" @default.
- W3204151063 cites W1490231232 @default.
- W3204151063 cites W1502713173 @default.
- W3204151063 cites W1503311137 @default.
- W3204151063 cites W1528634627 @default.
- W3204151063 cites W1533942477 @default.
- W3204151063 cites W1558348929 @default.
- W3204151063 cites W1588320408 @default.
- W3204151063 cites W1649026641 @default.
- W3204151063 cites W1770731112 @default.
- W3204151063 cites W190377005 @default.
- W3204151063 cites W1969670934 @default.
- W3204151063 cites W1971305813 @default.
- W3204151063 cites W1973260880 @default.
- W3204151063 cites W1977709885 @default.
- W3204151063 cites W1979873290 @default.
- W3204151063 cites W1980744841 @default.
- W3204151063 cites W1981570640 @default.
- W3204151063 cites W1985846539 @default.
- W3204151063 cites W1989421786 @default.
- W3204151063 cites W1989467586 @default.
- W3204151063 cites W1990149668 @default.
- W3204151063 cites W1993627126 @default.
- W3204151063 cites W2000134265 @default.
- W3204151063 cites W2003171404 @default.
- W3204151063 cites W2004078197 @default.
- W3204151063 cites W2004596155 @default.
- W3204151063 cites W2008610647 @default.
- W3204151063 cites W2011556073 @default.
- W3204151063 cites W2012916279 @default.
- W3204151063 cites W2016197401 @default.
- W3204151063 cites W2016441400 @default.
- W3204151063 cites W2018191400 @default.
- W3204151063 cites W2018338317 @default.
- W3204151063 cites W2018836029 @default.
- W3204151063 cites W2024088186 @default.
- W3204151063 cites W2024518588 @default.
- W3204151063 cites W2031211461 @default.
- W3204151063 cites W2036079113 @default.
- W3204151063 cites W2037710811 @default.
- W3204151063 cites W2038449573 @default.
- W3204151063 cites W2040474954 @default.
- W3204151063 cites W2042087070 @default.
- W3204151063 cites W2044453460 @default.
- W3204151063 cites W2046241907 @default.
- W3204151063 cites W2048263097 @default.
- W3204151063 cites W2048834720 @default.
- W3204151063 cites W2051536567 @default.
- W3204151063 cites W2051721448 @default.
- W3204151063 cites W2053524220 @default.
- W3204151063 cites W2055043387 @default.
- W3204151063 cites W2056314801 @default.
- W3204151063 cites W2056607606 @default.
- W3204151063 cites W2057451703 @default.
- W3204151063 cites W2058487126 @default.
- W3204151063 cites W2064390292 @default.
- W3204151063 cites W2070021921 @default.
- W3204151063 cites W2071445941 @default.
- W3204151063 cites W2074918990 @default.
- W3204151063 cites W2076154138 @default.
- W3204151063 cites W2077381938 @default.
- W3204151063 cites W2080324830 @default.
- W3204151063 cites W2080336014 @default.
- W3204151063 cites W2082401264 @default.
- W3204151063 cites W2086730810 @default.
- W3204151063 cites W2087083968 @default.
- W3204151063 cites W2091414779 @default.
- W3204151063 cites W2093443640 @default.
- W3204151063 cites W2094480378 @default.
- W3204151063 cites W2100531260 @default.
- W3204151063 cites W2101491950 @default.
- W3204151063 cites W2102619694 @default.
- W3204151063 cites W2107279676 @default.
- W3204151063 cites W2107959600 @default.
- W3204151063 cites W2108234281 @default.
- W3204151063 cites W2108797218 @default.
- W3204151063 cites W2108963581 @default.
- W3204151063 cites W2114599216 @default.
- W3204151063 cites W2114716625 @default.
- W3204151063 cites W2115603949 @default.
- W3204151063 cites W2118901290 @default.
- W3204151063 cites W2119787118 @default.
- W3204151063 cites W2122081519 @default.
- W3204151063 cites W2127081952 @default.
- W3204151063 cites W2127862779 @default.
- W3204151063 cites W2130198345 @default.
- W3204151063 cites W2131034808 @default.
- W3204151063 cites W2131180975 @default.
- W3204151063 cites W2131374955 @default.
- W3204151063 cites W2132199053 @default.
- W3204151063 cites W2139122477 @default.
- W3204151063 cites W2142853677 @default.
- W3204151063 cites W2150194502 @default.
- W3204151063 cites W2150914815 @default.