Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204170037> ?p ?o ?g. }
- W3204170037 abstract "Abstract Cryopreservation is essential for the long‐term storage of primary cell samples and enables accessing primary cells anytime via a simple thawing process. However, low viability and cell fragmentation are the major issues for cryopreserved cell samples due to the toxicity of cryoprotective agents and crystallization of intracellular contents during cooling at low temperature. Human peripheral blood mononuclear cells (PBMCs) in high viability are critical for pathophysiological and therapeutic research of infectious diseases, especially for the current worldwide pandemic of coronavirus disease 2019. Conventional viability enrichment techniques for primary PBMC samples provoke either cryoinjury caused by cell sorting mechanism or substantial cell loss due to multiple sample preparation steps. Here, a label‐free viability assessment and on‐demand enrichment system for cryopreserved primary human PBMCs with validation by fluorescent label‐based flow cytometry, is demonstrated. It can provide consistent viability assays by discriminating viable PBMCs from apoptotic, DMSO toxicity‐induced dead cells and cell debris, and deliver over 90% viability of sorted samples in one step after thawing. This integrated cell viability assay and on‐demand enrichment of viable cells has broad applications in diverse cellular biology research and cell‐related biomedical diagnostics." @default.
- W3204170037 created "2021-10-11" @default.
- W3204170037 creator A5011514006 @default.
- W3204170037 creator A5018545870 @default.
- W3204170037 creator A5076836770 @default.
- W3204170037 creator A5081022670 @default.
- W3204170037 date "2021-10-03" @default.
- W3204170037 modified "2023-10-18" @default.
- W3204170037 title "Label‐Free Cell Viability Assay and Enrichment of Cryopreserved Cells Using Microfluidic Cytometry and On‐Demand Sorting" @default.
- W3204170037 cites W1500925296 @default.
- W3204170037 cites W159995254 @default.
- W3204170037 cites W1970101659 @default.
- W3204170037 cites W1974281256 @default.
- W3204170037 cites W1977248858 @default.
- W3204170037 cites W1985039060 @default.
- W3204170037 cites W1989218872 @default.
- W3204170037 cites W1997713834 @default.
- W3204170037 cites W1998248235 @default.
- W3204170037 cites W2009977841 @default.
- W3204170037 cites W2017204918 @default.
- W3204170037 cites W2019072696 @default.
- W3204170037 cites W2028338939 @default.
- W3204170037 cites W2034100391 @default.
- W3204170037 cites W2047409754 @default.
- W3204170037 cites W2049812405 @default.
- W3204170037 cites W2068127452 @default.
- W3204170037 cites W2075468711 @default.
- W3204170037 cites W2081507989 @default.
- W3204170037 cites W2091124606 @default.
- W3204170037 cites W2091296592 @default.
- W3204170037 cites W2093471054 @default.
- W3204170037 cites W2112738559 @default.
- W3204170037 cites W2116070039 @default.
- W3204170037 cites W2118206169 @default.
- W3204170037 cites W2120629404 @default.
- W3204170037 cites W2125951290 @default.
- W3204170037 cites W2189647639 @default.
- W3204170037 cites W2279624096 @default.
- W3204170037 cites W2297613851 @default.
- W3204170037 cites W2512482408 @default.
- W3204170037 cites W2571352594 @default.
- W3204170037 cites W2743492062 @default.
- W3204170037 cites W2791833314 @default.
- W3204170037 cites W2868963225 @default.
- W3204170037 cites W2951589241 @default.
- W3204170037 cites W2988813558 @default.
- W3204170037 cites W3004895699 @default.
- W3204170037 cites W3004922691 @default.
- W3204170037 cites W3015830451 @default.
- W3204170037 cites W3021575042 @default.
- W3204170037 cites W3043029189 @default.
- W3204170037 cites W3043831218 @default.
- W3204170037 cites W3085293961 @default.
- W3204170037 cites W3089288413 @default.
- W3204170037 cites W3093388579 @default.
- W3204170037 cites W3093443066 @default.
- W3204170037 cites W3094282922 @default.
- W3204170037 cites W3094320129 @default.
- W3204170037 cites W3112334210 @default.
- W3204170037 cites W3127756616 @default.
- W3204170037 cites W3127935100 @default.
- W3204170037 cites W3129126066 @default.
- W3204170037 cites W3129273761 @default.
- W3204170037 cites W3129999347 @default.
- W3204170037 cites W3131610184 @default.
- W3204170037 cites W3174741326 @default.
- W3204170037 cites W865043859 @default.
- W3204170037 doi "https://doi.org/10.1002/admt.202100906" @default.
- W3204170037 hasPublicationYear "2021" @default.
- W3204170037 type Work @default.
- W3204170037 sameAs 3204170037 @default.
- W3204170037 citedByCount "9" @default.
- W3204170037 countsByYear W32041700372022 @default.
- W3204170037 countsByYear W32041700372023 @default.
- W3204170037 crossrefType "journal-article" @default.
- W3204170037 hasAuthorship W3204170037A5011514006 @default.
- W3204170037 hasAuthorship W3204170037A5018545870 @default.
- W3204170037 hasAuthorship W3204170037A5076836770 @default.
- W3204170037 hasAuthorship W3204170037A5081022670 @default.
- W3204170037 hasConcept C137061746 @default.
- W3204170037 hasConcept C1491633281 @default.
- W3204170037 hasConcept C179933525 @default.
- W3204170037 hasConcept C196843134 @default.
- W3204170037 hasConcept C202751555 @default.
- W3204170037 hasConcept C203014093 @default.
- W3204170037 hasConcept C2778630268 @default.
- W3204170037 hasConcept C2780339063 @default.
- W3204170037 hasConcept C53227056 @default.
- W3204170037 hasConcept C553184892 @default.
- W3204170037 hasConcept C55493867 @default.
- W3204170037 hasConcept C86803240 @default.
- W3204170037 hasConcept C95444343 @default.
- W3204170037 hasConceptScore W3204170037C137061746 @default.
- W3204170037 hasConceptScore W3204170037C1491633281 @default.
- W3204170037 hasConceptScore W3204170037C179933525 @default.
- W3204170037 hasConceptScore W3204170037C196843134 @default.
- W3204170037 hasConceptScore W3204170037C202751555 @default.
- W3204170037 hasConceptScore W3204170037C203014093 @default.
- W3204170037 hasConceptScore W3204170037C2778630268 @default.
- W3204170037 hasConceptScore W3204170037C2780339063 @default.