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- W3204192643 abstract "Abstract Recent work has revealed that spontaneous release plays critical roles in the central nervous system, but how it is regulated remains elusive. Here, we report that synaptotagmin‐11 (Syt11), a Ca 2+ ‐independent Syt isoform associated with schizophrenia and Parkinson's disease, suppressed spontaneous release. Syt11‐knockout hippocampal neurons showed an increased frequency of miniature excitatory post‐synaptic currents while over‐expression of Syt11 inversely decreased the frequency. Neither knockout nor over‐expression of Syt11 affected the average amplitude, suggesting the pre‐synaptic regulation of spontaneous neurotransmission by Syt11. Glutathione S‐transferase pull‐down, co‐immunoprecipitation, and affinity‐purification experiments demonstrated a direct interaction of Syt11 with vps10p‐tail‐interactor‐1a (vti1a), a non‐canonical SNARE protein that maintains spontaneous release. Importantly, knockdown of vti1a reversed the phenotype of Syt11 knockout, identifying vti1a as the main target of Syt11 inhibition. Domain analysis revealed that the C2A domain of Syt11 bound vti1a with high affinity. Consistently, expression of the C2A domain alone rescued the phenotype of elevated spontaneous release in Syt11‐knockout neurons similar to the full‐length protein. Altogether, our results suggest that Syt11 inhibits vti1a‐containing vesicles during spontaneous release. image" @default.
- W3204192643 created "2021-10-11" @default.
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- W3204192643 date "2021-10-14" @default.
- W3204192643 modified "2023-10-06" @default.
- W3204192643 title "Synaptotagmin‐11 inhibits spontaneous neurotransmission through vti1a" @default.
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- W3204192643 cites W1980682773 @default.
- W3204192643 cites W1981726470 @default.
- W3204192643 cites W1983154767 @default.
- W3204192643 cites W1989232733 @default.
- W3204192643 cites W1991546697 @default.
- W3204192643 cites W1997090186 @default.
- W3204192643 cites W1997671401 @default.
- W3204192643 cites W1998116909 @default.
- W3204192643 cites W1998573306 @default.
- W3204192643 cites W1999639946 @default.
- W3204192643 cites W2003674836 @default.
- W3204192643 cites W2003748392 @default.
- W3204192643 cites W2008456025 @default.
- W3204192643 cites W2019558341 @default.
- W3204192643 cites W2019766891 @default.
- W3204192643 cites W2024136850 @default.
- W3204192643 cites W2028800381 @default.
- W3204192643 cites W2029191046 @default.
- W3204192643 cites W2032240583 @default.
- W3204192643 cites W2032840486 @default.
- W3204192643 cites W2034038842 @default.
- W3204192643 cites W2037168572 @default.
- W3204192643 cites W2041423137 @default.
- W3204192643 cites W2042212641 @default.
- W3204192643 cites W2045279519 @default.
- W3204192643 cites W2046369372 @default.
- W3204192643 cites W2058035029 @default.
- W3204192643 cites W2060012772 @default.
- W3204192643 cites W2063286825 @default.
- W3204192643 cites W2074235051 @default.
- W3204192643 cites W2075858010 @default.
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- W3204192643 cites W2102492718 @default.
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- W3204192643 cites W2116895182 @default.
- W3204192643 cites W2121235255 @default.
- W3204192643 cites W2126028696 @default.
- W3204192643 cites W2132653427 @default.
- W3204192643 cites W2136422440 @default.
- W3204192643 cites W2143300213 @default.
- W3204192643 cites W2144447780 @default.
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- W3204192643 cites W2218722008 @default.
- W3204192643 cites W2262173945 @default.
- W3204192643 cites W2411516205 @default.
- W3204192643 cites W2550506547 @default.
- W3204192643 cites W2587213236 @default.
- W3204192643 cites W2728889421 @default.
- W3204192643 cites W2735752438 @default.
- W3204192643 cites W2744914260 @default.
- W3204192643 cites W2766189988 @default.
- W3204192643 cites W2771811251 @default.
- W3204192643 cites W2782239432 @default.
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- W3204192643 doi "https://doi.org/10.1111/jnc.15523" @default.
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