Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204195432> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W3204195432 abstract "Abstract Microbial communities occupy diverse niches in nature, and exchanges of metabolites such as carbon sources, amino acids, and vitamins occur routinely among the community members. While large-scale metagenomic and metabolomic studies shed some light on these exchanges, the contribution of individual species and the molecular details of specific interactions are difficult to track. Here, we explore the molecular picture of vitamin B 1 (thiamin) metabolism occurring in synthetic communities of Escherichia coli thiamin auxotrophs which engage in the exchange of thiamin and its biosynthesis intermediates. In E. coli , the two parts of thiamin – the 4-amino-5-hydroxymethyl-2-methylpyrimidine and the 4-methyl-5-(2-hydroxyethyl)thiazole – are synthesized by separate pathways using enzymes ThiC and ThiG, respectively, and are then joined by ThiE to form thiamin. We observed that even though E. coli ΔthiC, ΔthiE , and ΔthiG mutants are thiamin auxotrophs, co-cultures of ΔthiC - ΔthiE and ΔthiC - ΔthiG grow in a thiamin-deficient minimal medium, whereas the ΔthiE - ΔthiG co-culture does not. Analysis of the exchange of thiamin and its intermediates in Vibrio anguillarum co-cultures, and in mixed co-cultures of V. anguillarum and E. coli revealed that the general pattern of thiamin metabolism and exchange among microbes is conserved across species. Specifically, the microorganisms exchange HMP and thiamin easily among themselves but not THZ. Furthermore, we observe that the availability of exogenous thiamin in the media affects whether these strains interact with each other or grow independently. This underscores the importance of the exchange of essential metabolites as a defining factor in building and modulating synthetic or natural microbial communities." @default.
- W3204195432 created "2021-10-11" @default.
- W3204195432 creator A5001163496 @default.
- W3204195432 creator A5026236443 @default.
- W3204195432 creator A5040581317 @default.
- W3204195432 date "2021-09-29" @default.
- W3204195432 modified "2023-10-14" @default.
- W3204195432 title "The exchange of vitamin B1 and its biosynthesis intermediates in synthetic microbial communities shapes the community composition and reveals complexities of nutrient sharing" @default.
- W3204195432 cites W1579467601 @default.
- W3204195432 cites W1894075467 @default.
- W3204195432 cites W1989864831 @default.
- W3204195432 cites W1996016026 @default.
- W3204195432 cites W2006480444 @default.
- W3204195432 cites W2018672567 @default.
- W3204195432 cites W2025136839 @default.
- W3204195432 cites W2025538655 @default.
- W3204195432 cites W2056782669 @default.
- W3204195432 cites W2068481735 @default.
- W3204195432 cites W2079946180 @default.
- W3204195432 cites W2082308349 @default.
- W3204195432 cites W2101399358 @default.
- W3204195432 cites W2110347270 @default.
- W3204195432 cites W2111967267 @default.
- W3204195432 cites W2113350312 @default.
- W3204195432 cites W2114684949 @default.
- W3204195432 cites W2116137883 @default.
- W3204195432 cites W2116524545 @default.
- W3204195432 cites W2120321004 @default.
- W3204195432 cites W2126999541 @default.
- W3204195432 cites W2168383728 @default.
- W3204195432 cites W2169010693 @default.
- W3204195432 cites W2225078051 @default.
- W3204195432 cites W2402553767 @default.
- W3204195432 cites W2560034796 @default.
- W3204195432 cites W2586852705 @default.
- W3204195432 cites W2758795952 @default.
- W3204195432 cites W2763538746 @default.
- W3204195432 cites W2794647037 @default.
- W3204195432 cites W2797164697 @default.
- W3204195432 cites W2804100011 @default.
- W3204195432 cites W2804854320 @default.
- W3204195432 cites W2891280052 @default.
- W3204195432 cites W2897257450 @default.
- W3204195432 cites W2906762386 @default.
- W3204195432 cites W2950537876 @default.
- W3204195432 cites W2951076868 @default.
- W3204195432 cites W2964309768 @default.
- W3204195432 cites W3012893048 @default.
- W3204195432 cites W3117862598 @default.
- W3204195432 doi "https://doi.org/10.1101/2021.09.29.462401" @default.
- W3204195432 hasPublicationYear "2021" @default.
- W3204195432 type Work @default.
- W3204195432 sameAs 3204195432 @default.
- W3204195432 citedByCount "1" @default.
- W3204195432 countsByYear W32041954322023 @default.
- W3204195432 crossrefType "posted-content" @default.
- W3204195432 hasAuthorship W3204195432A5001163496 @default.
- W3204195432 hasAuthorship W3204195432A5026236443 @default.
- W3204195432 hasAuthorship W3204195432A5040581317 @default.
- W3204195432 hasBestOaLocation W32041954321 @default.
- W3204195432 hasConcept C104317684 @default.
- W3204195432 hasConcept C181199279 @default.
- W3204195432 hasConcept C2776203871 @default.
- W3204195432 hasConcept C547475151 @default.
- W3204195432 hasConcept C553450214 @default.
- W3204195432 hasConcept C55493867 @default.
- W3204195432 hasConcept C86803240 @default.
- W3204195432 hasConceptScore W3204195432C104317684 @default.
- W3204195432 hasConceptScore W3204195432C181199279 @default.
- W3204195432 hasConceptScore W3204195432C2776203871 @default.
- W3204195432 hasConceptScore W3204195432C547475151 @default.
- W3204195432 hasConceptScore W3204195432C553450214 @default.
- W3204195432 hasConceptScore W3204195432C55493867 @default.
- W3204195432 hasConceptScore W3204195432C86803240 @default.
- W3204195432 hasLocation W32041954321 @default.
- W3204195432 hasOpenAccess W3204195432 @default.
- W3204195432 hasPrimaryLocation W32041954321 @default.
- W3204195432 hasRelatedWork W1530599277 @default.
- W3204195432 hasRelatedWork W1587895966 @default.
- W3204195432 hasRelatedWork W2055236270 @default.
- W3204195432 hasRelatedWork W2080899182 @default.
- W3204195432 hasRelatedWork W2080910126 @default.
- W3204195432 hasRelatedWork W2111819350 @default.
- W3204195432 hasRelatedWork W2117469002 @default.
- W3204195432 hasRelatedWork W2320259508 @default.
- W3204195432 hasRelatedWork W2331680167 @default.
- W3204195432 hasRelatedWork W3021739321 @default.
- W3204195432 isParatext "false" @default.
- W3204195432 isRetracted "false" @default.
- W3204195432 magId "3204195432" @default.
- W3204195432 workType "article" @default.