FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204206907> ?p ?o ?g. }

• W3204206907 endingPage "e0258039" @default.
• W3204206907 startingPage "e0258039" @default.
• W3204206907 abstract "Soil-transmitted helminths (STH) infect up to one-quarter of the global population, with a significant associated disease burden. The main human STH are: Ancylostoma spp. and Necator americanus (hookworms); Ascaris lumbricoides , Trichuris trichiura , and Strongyloides stercoralis . The aim of this study was to establish a scalable system for stool STH multiplex quantitative real-time polymerase chain reactions (qPCR). Stool samples collected in Fiji and preserved in potassium dichromate were transferred to Melbourne at ambient temperature. Samples were washed to remove potassium dichromate and DNA was extracted with the Mini-Beadbeater-24 and a column-based kit. A SYBR green qPCR to detect the vertebrate mitochondrial gene was used as a DNA extraction control. Samples were tested using a probe-based multiplex qPCR targeting A . lumbricoides , T . trichiura and S . stercoralis , and in a second multiplex reaction to detect hookworms to the species level ( A . duodenale , A . ceylanicum , N . americanus ). An internal amplification control in both multiplex assays was included to prevent false-negative results due to PCR inhibitors. Samples were homogenised for a single cycle of 40 seconds to release STH DNA and washed stool was stored for up to 15 weeks at -30°C without compromising DNA. Our multiplex qPCR detected multiple species of STH without reduced sensitivity compared to singleplex. qPCR data from 40 stools was validated against STH-positive stools determined by microscopy. We have developed and validated an efficient and staged system for detecting six clinically important STH affecting humans that could be easily implemented without advanced automation in any qPCR-capable laboratory." @default.
• W3204206907 created "2021-10-11" @default.
• W3204206907 creator A5021130202 @default.
• W3204206907 creator A5038847729 @default.
• W3204206907 creator A5042165812 @default.
• W3204206907 creator A5049716200 @default.
• W3204206907 creator A5050964637 @default.
• W3204206907 creator A5055957305 @default.
• W3204206907 creator A5069014035 @default.
• W3204206907 creator A5090268548 @default.
• W3204206907 date "2021-09-30" @default.
• W3204206907 modified "2023-09-25" @default.
• W3204206907 title "Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow" @default.
• W3204206907 cites W1579968872 @default.
• W3204206907 cites W1982782498 @default.
• W3204206907 cites W1992257126 @default.
• W3204206907 cites W1994175649 @default.
• W3204206907 cites W2001181252 @default.
• W3204206907 cites W2004261679 @default.
• W3204206907 cites W2006693262 @default.
• W3204206907 cites W2015290391 @default.
• W3204206907 cites W2027618731 @default.
• W3204206907 cites W2039303462 @default.
• W3204206907 cites W2040633924 @default.
• W3204206907 cites W2054964732 @default.
• W3204206907 cites W2066262604 @default.
• W3204206907 cites W2068537511 @default.
• W3204206907 cites W2070910723 @default.
• W3204206907 cites W2097953574 @default.
• W3204206907 cites W2100568594 @default.
• W3204206907 cites W2105614110 @default.
• W3204206907 cites W2106988468 @default.
• W3204206907 cites W2107920367 @default.
• W3204206907 cites W2123559199 @default.
• W3204206907 cites W2134199230 @default.
• W3204206907 cites W2140349011 @default.
• W3204206907 cites W2142662767 @default.
• W3204206907 cites W2153384793 @default.
• W3204206907 cites W2155380292 @default.
• W3204206907 cites W2162649979 @default.
• W3204206907 cites W2164357708 @default.
• W3204206907 cites W2166326554 @default.
• W3204206907 cites W2282285824 @default.
• W3204206907 cites W2607347173 @default.
• W3204206907 cites W2767411594 @default.
• W3204206907 cites W2785132065 @default.
• W3204206907 cites W2785281829 @default.
• W3204206907 cites W2810858791 @default.
• W3204206907 cites W2889005146 @default.
• W3204206907 cites W2891292359 @default.
• W3204206907 cites W2926993597 @default.
• W3204206907 cites W2946780516 @default.
• W3204206907 cites W2952011713 @default.
• W3204206907 cites W2982485123 @default.
• W3204206907 cites W3010883656 @default.
• W3204206907 cites W3028639237 @default.
• W3204206907 cites W3037628346 @default.
• W3204206907 cites W3133998939 @default.
• W3204206907 cites W3163969822 @default.
• W3204206907 doi "https://doi.org/10.1371/journal.pone.0258039" @default.
• W3204206907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8483301" @default.
• W3204206907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34591904" @default.
• W3204206907 hasPublicationYear "2021" @default.
• W3204206907 type Work @default.
• W3204206907 sameAs 3204206907 @default.
• W3204206907 citedByCount "3" @default.
• W3204206907 countsByYear W32042069072022 @default.
• W3204206907 crossrefType "journal-article" @default.
• W3204206907 hasAuthorship W3204206907A5021130202 @default.
• W3204206907 hasAuthorship W3204206907A5038847729 @default.
• W3204206907 hasAuthorship W3204206907A5042165812 @default.
• W3204206907 hasAuthorship W3204206907A5049716200 @default.
• W3204206907 hasAuthorship W3204206907A5050964637 @default.
• W3204206907 hasAuthorship W3204206907A5055957305 @default.
• W3204206907 hasAuthorship W3204206907A5069014035 @default.
• W3204206907 hasAuthorship W3204206907A5090268548 @default.
• W3204206907 hasBestOaLocation W32042069071 @default.
• W3204206907 hasConcept C104317684 @default.
• W3204206907 hasConcept C165901193 @default.
• W3204206907 hasConcept C203014093 @default.
• W3204206907 hasConcept C2776117458 @default.
• W3204206907 hasConcept C2777047024 @default.
• W3204206907 hasConcept C2777364306 @default.
• W3204206907 hasConcept C2777683361 @default.
• W3204206907 hasConcept C2778013117 @default.
• W3204206907 hasConcept C2780968160 @default.
• W3204206907 hasConcept C2781188995 @default.
• W3204206907 hasConcept C42972112 @default.
• W3204206907 hasConcept C48023723 @default.
• W3204206907 hasConcept C49105822 @default.
• W3204206907 hasConcept C54355233 @default.
• W3204206907 hasConcept C71924100 @default.
• W3204206907 hasConcept C78063203 @default.
• W3204206907 hasConcept C86803240 @default.
• W3204206907 hasConcept C89423630 @default.
• W3204206907 hasConcept C90583042 @default.
• W3204206907 hasConceptScore W3204206907C104317684 @default.
• W3204206907 hasConceptScore W3204206907C165901193 @default.

• next