FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204245114> ?p ?o ?g. }

• W3204245114 endingPage "410" @default.
• W3204245114 startingPage "400" @default.
• W3204245114 abstract "Sensitive and quantitative detection of molecular biomarkers is crucial for the early diagnosis of diseases like metabolic syndrome and cancer. Here we present a single-molecule sandwich immunoassay by imaging the number of single nanoparticles to diagnose aggressive prostate cancer. Our assay employed the photo-stable upconversion nanoparticles (UCNPs) as labels to detect the four types of circulating antigens in blood circulation, including glypican-1 (GPC-1), leptin, osteopontin (OPN), and vascular endothelial growth factor (VEGF), as their serum concentrations indicate aggressive prostate cancer. Under a wide-field microscope, a single UCNP doped with thousands of lanthanide ions can emit sufficiently bright anti-Stokes' luminescence to become quantitatively detectable. By counting every single streptavidin-functionalized UCNP which specifically labeled on each sandwich immune complex across multiple fields of views, we achieved the Limit of Detection (LOD) of 0.0123 ng/ml, 0.2711 ng/ml, 0.1238 ng/ml, and 0.0158 ng/ml for GPC-1, leptin, OPN and VEGF, respectively. The serum circulating level of GPC-1, leptin, OPN, and VEGF in a mixture of 10 healthy normal human serum was 25.17 ng/ml, 18.04 ng/ml, 11.34 ng/ml, and 1.55 ng/ml, which was within the assay dynamic detection range for each analyte. Moreover, a 20% increase of GPC-1 and OPN was observed by spiking the normal human serum with recombinant antigens to confirm the accuracy of the assay. We observed no cross-reactivity among the four biomarker analytes, which eliminates the false positives and enhances the detection accuracy. The developed single upconversion nanoparticle-assisted single-molecule assay suggests its potential in clinical usage for prostate cancer detection by monitoring tiny concentration differences in a panel of serum biomarkers." @default.
• W3204245114 created "2021-10-11" @default.
• W3204245114 creator A5016001628 @default.
• W3204245114 creator A5016607165 @default.
• W3204245114 creator A5016814335 @default.
• W3204245114 creator A5022637761 @default.
• W3204245114 creator A5025300404 @default.
• W3204245114 creator A5026383364 @default.
• W3204245114 creator A5029679296 @default.
• W3204245114 creator A5032015030 @default.
• W3204245114 creator A5040572278 @default.
• W3204245114 creator A5064837656 @default.
• W3204245114 creator A5075403679 @default.
• W3204245114 creator A5084159998 @default.
• W3204245114 creator A5086878223 @default.
• W3204245114 date "2021-10-03" @default.
• W3204245114 modified "2023-10-16" @default.
• W3204245114 title "Upconversion nanoparticle‐assisted single‐molecule assay for detecting circulating antigens of aggressive prostate cancer" @default.
• W3204245114 cites W1587220291 @default.
• W3204245114 cites W1819972003 @default.
• W3204245114 cites W1978059875 @default.
• W3204245114 cites W2017157952 @default.
• W3204245114 cites W2018691490 @default.
• W3204245114 cites W2024703652 @default.
• W3204245114 cites W2027230614 @default.
• W3204245114 cites W2035564393 @default.
• W3204245114 cites W2055624262 @default.
• W3204245114 cites W2080232019 @default.
• W3204245114 cites W2087654438 @default.
• W3204245114 cites W2137862731 @default.
• W3204245114 cites W2171098112 @default.
• W3204245114 cites W2270958371 @default.
• W3204245114 cites W2336450175 @default.
• W3204245114 cites W2487264607 @default.
• W3204245114 cites W2552503041 @default.
• W3204245114 cites W2589983485 @default.
• W3204245114 cites W2608293026 @default.
• W3204245114 cites W2757348710 @default.
• W3204245114 cites W2765079756 @default.
• W3204245114 cites W2773653115 @default.
• W3204245114 cites W2784902308 @default.
• W3204245114 cites W2789210622 @default.
• W3204245114 cites W2795977202 @default.
• W3204245114 cites W2799413022 @default.
• W3204245114 cites W2808632617 @default.
• W3204245114 cites W2886427651 @default.
• W3204245114 cites W2886548961 @default.
• W3204245114 cites W2891807736 @default.
• W3204245114 cites W2896106860 @default.
• W3204245114 cites W2951065479 @default.
• W3204245114 cites W2955144726 @default.
• W3204245114 cites W2964765396 @default.
• W3204245114 cites W2968872684 @default.
• W3204245114 cites W2994455805 @default.
• W3204245114 cites W3000616687 @default.
• W3204245114 cites W3001874096 @default.
• W3204245114 cites W3009337487 @default.
• W3204245114 cites W3013621908 @default.
• W3204245114 cites W3016843709 @default.
• W3204245114 cites W3109716138 @default.
• W3204245114 cites W3118256895 @default.
• W3204245114 cites W3130963652 @default.
• W3204245114 cites W3141906757 @default.
• W3204245114 cites W3163469819 @default.
• W3204245114 cites W3193334186 @default.
• W3204245114 cites W797404722 @default.
• W3204245114 doi "https://doi.org/10.1002/cyto.a.24504" @default.
• W3204245114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34585823" @default.
• W3204245114 hasPublicationYear "2021" @default.
• W3204245114 type Work @default.
• W3204245114 sameAs 3204245114 @default.
• W3204245114 citedByCount "5" @default.
• W3204245114 countsByYear W32042451142022 @default.
• W3204245114 countsByYear W32042451142023 @default.
• W3204245114 crossrefType "journal-article" @default.
• W3204245114 hasAuthorship W3204245114A5016001628 @default.
• W3204245114 hasAuthorship W3204245114A5016607165 @default.
• W3204245114 hasAuthorship W3204245114A5016814335 @default.
• W3204245114 hasAuthorship W3204245114A5022637761 @default.
• W3204245114 hasAuthorship W3204245114A5025300404 @default.
• W3204245114 hasAuthorship W3204245114A5026383364 @default.
• W3204245114 hasAuthorship W3204245114A5029679296 @default.
• W3204245114 hasAuthorship W3204245114A5032015030 @default.
• W3204245114 hasAuthorship W3204245114A5040572278 @default.
• W3204245114 hasAuthorship W3204245114A5064837656 @default.
• W3204245114 hasAuthorship W3204245114A5075403679 @default.
• W3204245114 hasAuthorship W3204245114A5084159998 @default.
• W3204245114 hasAuthorship W3204245114A5086878223 @default.
• W3204245114 hasConcept C10390740 @default.
• W3204245114 hasConcept C119128265 @default.
• W3204245114 hasConcept C121608353 @default.
• W3204245114 hasConcept C126322002 @default.
• W3204245114 hasConcept C145802702 @default.
• W3204245114 hasConcept C147483822 @default.
• W3204245114 hasConcept C153911025 @default.
• W3204245114 hasConcept C159654299 @default.
• W3204245114 hasConcept C185592680 @default.
• W3204245114 hasConcept C203014093 @default.

• next