Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204253085> ?p ?o ?g. }
- W3204253085 abstract "The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA. RecBCD is a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks. It also facilitates coating of single-stranded DNA with RecA to form RecA filaments, a vital step in the double-stranded break DNA repair pathway. However, questions remain about the mechanistic roles of RecA and RecBCD in live cells. Here, we use millisecond super-resolved fluorescence microscopy to pinpoint the spatial localization of fluorescent reporters of RecA or RecB at physiological levels of expression in individual live Escherichia coli cells. By introducing the DNA cross-linker mitomycin C, we induce DNA damage and quantify the resulting steady state changes in stoichiometry, cellular protein copy number and molecular mobilities of RecA and RecB. We find that both proteins accumulate in molecular hotspots to effect repair, resulting in RecA stoichiometries equivalent to several hundred molecules that assemble largely in dimeric subunits before DNA damage, but form periodic subunits of approximately 3-4 molecules within mature filaments of several thousand molecules. Unexpectedly, we find that the physiologically predominant forms of RecB are not only rapidly diffusing monomers, but slowly diffusing dimers." @default.
- W3204253085 created "2021-10-11" @default.
- W3204253085 creator A5006014233 @default.
- W3204253085 creator A5020568316 @default.
- W3204253085 creator A5033374272 @default.
- W3204253085 creator A5035067312 @default.
- W3204253085 creator A5074311677 @default.
- W3204253085 date "2022-08-01" @default.
- W3204253085 modified "2023-10-14" @default.
- W3204253085 title "RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live <i>Escherichia coli</i> with single-molecule sensitivity" @default.
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