Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204283930> ?p ?o ?g. }
- W3204283930 abstract "Summary Modular methods for directing mammalian gene expression would enable advances in tissue regeneration, enhance cell-based therapeutics and improve modulation of immune responses. To address this challenge, engineered endosymbionts (EES) that escape endosomal destruction, reside in the cytoplasm of mammalian cells, and secrete proteins that are transported to the nucleus to control host cell gene expression were developed. Microscopy confirmed that EES escape phagosomes, replicate within the cytoplasm, and can secrete reporter proteins into the cytoplasm that were then transported to the nucleus. Synthetic operons encoding the mammalian transcription factors, Stat-1 and Klf6 or Klf4 and Gata-3 were recombined into the EES genome. Using controlled induction, these EES were shown to direct gene expression in J774A.1 macrophage/monocyte cells and modulate the host cell fates. Expressing mammalian transcription factors from engineered intracellular bacteria as endosymbionts comprises a new tool for directing host cell gene expression for therapeutic and research purposes. Graphical abstract" @default.
- W3204283930 created "2021-10-11" @default.
- W3204283930 creator A5049939267 @default.
- W3204283930 creator A5050627390 @default.
- W3204283930 creator A5059174425 @default.
- W3204283930 creator A5073644541 @default.
- W3204283930 creator A5081671050 @default.
- W3204283930 date "2021-10-06" @default.
- W3204283930 modified "2023-10-16" @default.
- W3204283930 title "Engineered endosymbionts capable of directing mammalian cell gene expression" @default.
- W3204283930 cites W146312067 @default.
- W3204283930 cites W1501402501 @default.
- W3204283930 cites W1531355433 @default.
- W3204283930 cites W1582834972 @default.
- W3204283930 cites W1697053366 @default.
- W3204283930 cites W1895934305 @default.
- W3204283930 cites W1970011982 @default.
- W3204283930 cites W1980042305 @default.
- W3204283930 cites W1981473578 @default.
- W3204283930 cites W1983220630 @default.
- W3204283930 cites W1983523188 @default.
- W3204283930 cites W1993018547 @default.
- W3204283930 cites W1998733027 @default.
- W3204283930 cites W2004428166 @default.
- W3204283930 cites W2011083779 @default.
- W3204283930 cites W2012006993 @default.
- W3204283930 cites W2017001156 @default.
- W3204283930 cites W2017749666 @default.
- W3204283930 cites W2020744745 @default.
- W3204283930 cites W2027768860 @default.
- W3204283930 cites W2030513350 @default.
- W3204283930 cites W2033722975 @default.
- W3204283930 cites W2034178032 @default.
- W3204283930 cites W2037510579 @default.
- W3204283930 cites W2048674598 @default.
- W3204283930 cites W2052828164 @default.
- W3204283930 cites W2054472394 @default.
- W3204283930 cites W2054540776 @default.
- W3204283930 cites W2055348159 @default.
- W3204283930 cites W2058567994 @default.
- W3204283930 cites W2058631111 @default.
- W3204283930 cites W2076833412 @default.
- W3204283930 cites W2087636605 @default.
- W3204283930 cites W2089881635 @default.
- W3204283930 cites W2090270199 @default.
- W3204283930 cites W2093705034 @default.
- W3204283930 cites W2101413510 @default.
- W3204283930 cites W2111086397 @default.
- W3204283930 cites W2126811287 @default.
- W3204283930 cites W2130946949 @default.
- W3204283930 cites W2145611227 @default.
- W3204283930 cites W2147015873 @default.
- W3204283930 cites W2168669893 @default.
- W3204283930 cites W2301038687 @default.
- W3204283930 cites W2418981018 @default.
- W3204283930 cites W2462130533 @default.
- W3204283930 cites W2523171917 @default.
- W3204283930 cites W2614841288 @default.
- W3204283930 cites W2620734517 @default.
- W3204283930 cites W2747149289 @default.
- W3204283930 cites W2755653587 @default.
- W3204283930 cites W2774643301 @default.
- W3204283930 cites W2779872789 @default.
- W3204283930 cites W2794433535 @default.
- W3204283930 cites W2804005571 @default.
- W3204283930 cites W2806694951 @default.
- W3204283930 cites W2887320959 @default.
- W3204283930 cites W2891959956 @default.
- W3204283930 cites W2893124025 @default.
- W3204283930 cites W2899469895 @default.
- W3204283930 cites W2901250091 @default.
- W3204283930 cites W2911989429 @default.
- W3204283930 cites W2919429069 @default.
- W3204283930 cites W2921242268 @default.
- W3204283930 cites W2925472932 @default.
- W3204283930 cites W2968188013 @default.
- W3204283930 cites W2982346128 @default.
- W3204283930 cites W2990455925 @default.
- W3204283930 cites W2993867165 @default.
- W3204283930 cites W2994722600 @default.
- W3204283930 cites W3004850823 @default.
- W3204283930 cites W3010345790 @default.
- W3204283930 cites W3021841191 @default.
- W3204283930 cites W3037899313 @default.
- W3204283930 cites W3041877569 @default.
- W3204283930 cites W3087406471 @default.
- W3204283930 cites W3091659787 @default.
- W3204283930 cites W3092086279 @default.
- W3204283930 cites W3092756473 @default.
- W3204283930 cites W3104517924 @default.
- W3204283930 cites W3111904808 @default.
- W3204283930 cites W3113648827 @default.
- W3204283930 cites W3124344026 @default.
- W3204283930 cites W3124631812 @default.
- W3204283930 cites W4211201091 @default.
- W3204283930 doi "https://doi.org/10.1101/2021.10.05.463266" @default.
- W3204283930 hasPublicationYear "2021" @default.
- W3204283930 type Work @default.
- W3204283930 sameAs 3204283930 @default.
- W3204283930 citedByCount "0" @default.