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- W3204407498 abstract "本试验旨在探究脱氧雪腐烯醇(DON)对牛卵母细胞体外成熟发育的影响及其作用机制。将牛卵丘卵母细胞复合体分别在含DON浓度为0、50、250、500、1 000 ng·mL-1的体外成熟培养液中进行体外成熟，检测卵丘细胞扩展程度及第一极体排出率，构建DON毒性模型。然后，借助此模型研究DON对卵母细胞的线粒体分布及后续受精卵裂率、囊胚发育率的影响，探究DON对牛卵母细胞体外成熟及后续发育能力的影响；通过检测体外成熟卵母细胞内的氧化相关因子(ROS、GSH)水平和抗氧化基因CAT、GPx4的mRNA表达量，揭示DON影响牛卵母细胞体外发育能力的分子机制。结果表明，250、500 ng·mL-1的DON显著抑制卵母细胞的第一极体排出(P < 0.05)，1 000 ng·mL-1的DON极显著抑制第一极体排出(P < 0.01)；250 ng·mL-1的DON显著抑制卵丘卵母细胞扩展(P < 0.05)，500、1 000 ng·mL-1的DON极显著抑制卵丘细胞扩展(P < 0.01)；后续试验选取DON浓度为500 ng·mL-1作为毒性模型(DON组)，与不含DON组(对照组)进行比较研究，结果发现，对照组与DON组的线粒体均匀分布比例(60.2%vs.40.0%)存在显著差异(P < 0.05)；试验组较对照组的受精卵裂率(33.6±3.6%vs.(67.7±2.6)%)及早期囊胚率((0.0±0.0)%vs.(18.3±2.2)%)均显著降低(P < 0.05)；试验组较对照组，卵母细胞内ROS水平(1.6 vs.1.0)显著升高(P < 0.05)，GSH相对水平(0.4 vs.1.0)显著降低(P < 0.05)，抗氧化基因CAT与GPx4的mRNA相对表达量(0.0 vs.1.0；0.6 vs.1.0)显著降低(P < 0.05)。以上研究表明，DON对卵母细胞的体外成熟及早期胚胎发育具有抑制作用，其作用机制与DON破坏牛卵母细胞内抗氧化系统平衡相关。" @default.
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- W3204407498 date "2021-09-23" @default.
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- W3204407498 title "脱氧雪腐烯醇(DON)对牛卵母细胞体外成熟及发育能力的影响" @default.
- W3204407498 doi "https://doi.org/10.11843/j.issn.0366-6964.2021.09.010" @default.
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