Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204425616> ?p ?o ?g. }
- W3204425616 abstract "It is essential for cells to control which genes are transcribed into RNA. In eukaryotes, two major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and subsequent pause release toward transcription. Pol II recruitment and pause release occur in association with macromolecular clusters, which were proposed to be formed by a liquid-liquid phase separation mechanism. How such a phase separation mechanism relates to the interaction of Pol II with DNA during recruitment and transcription, however, remains poorly understood. Here, we use live and super-resolution microscopy in zebrafish embryos to reveal Pol II clusters with a large variety of shapes, which can be explained by a theoretical model in which regulatory chromatin regions provide surfaces for liquid-phase condensation at concentrations that are too low for canonical liquid-liquid phase separation. Model simulations and chemical perturbation experiments indicate that recruited Pol II contributes to the formation of these surface-associated condensates, whereas elongating Pol II is excluded from these condensates and thereby drives their unfolding." @default.
- W3204425616 created "2021-10-11" @default.
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- W3204425616 date "2021-09-01" @default.
- W3204425616 modified "2023-10-15" @default.
- W3204425616 title "RNA polymerase II clusters form in line with surface condensation on regulatory chromatin" @default.
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