FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204477323> ?p ?o ?g. }

• W3204477323 endingPage "85" @default.
• W3204477323 startingPage "76" @default.
• W3204477323 abstract "Neutrophil extracellular traps increase cystic fibrosis (CF) airway inflammation. We hypothesized that macrophage exposure to neutrophil elastase (NE) would trigger the release of macrophage extracellular traps (METs), a novel mechanism to augment NE-induced airway inflammation in CF. Experiments were performed using human blood monocyte derived macrophages (hBMDM) from patients with and without CF to test specific mechanisms associated with MET release, and MET release by NE was confirmed in alveolar macrophages from Cftr-null and wild-type littermate mice exposed to intratracheal NE in vivo. Human BMDM were exposed to FITC-NE, and intracellular FITC-NE was localized to cytoplasmic and nuclear domains. Intracellular NE was proteolytically active as indicated by DQ-Elastin substrate cleavage. NE (100 to 500 nM) significantly increased extracellular PicoGreen fluorescence consistent with DNA release/ MET release from hBMDM in the absence of cell death. MET release was further confirmed by confocal microscopy in hBMDM treated with NE, and in alveolar macrophages from Cftr-null and wild-type littermate mice that had been exposed to intratracheal NE. NE-triggered MET release was associated with H3 citrullination detected by immunofluorescence assays and with partial cleavage of histone H3 but not H4. Exposure to NE caused release of METs from both CF and non-CF hBMDM in vitro and murine alveolar macrophages in vivo. MET release was associated with NE-activated H3 clipping, a mechanism associated with chromatin decondensation, a prerequisite for METs." @default.
• W3204477323 created "2021-10-11" @default.
• W3204477323 creator A5017217713 @default.
• W3204477323 creator A5021664553 @default.
• W3204477323 creator A5021974562 @default.
• W3204477323 creator A5044002777 @default.
• W3204477323 creator A5073196705 @default.
• W3204477323 creator A5083435207 @default.
• W3204477323 date "2022-01-01" @default.
• W3204477323 modified "2023-10-17" @default.
• W3204477323 title "Neutrophil Elastase Triggers the Release of Macrophage Extracellular Traps: Relevance to Cystic Fibrosis" @default.
• W3204477323 cites W1494334863 @default.
• W3204477323 cites W1603118564 @default.
• W3204477323 cites W1630708674 @default.
• W3204477323 cites W1702374914 @default.
• W3204477323 cites W1834210733 @default.
• W3204477323 cites W1975572445 @default.
• W3204477323 cites W1983738441 @default.
• W3204477323 cites W1995801005 @default.
• W3204477323 cites W2005293824 @default.
• W3204477323 cites W2011832904 @default.
• W3204477323 cites W2018860370 @default.
• W3204477323 cites W2031312929 @default.
• W3204477323 cites W2049470707 @default.
• W3204477323 cites W2058829322 @default.
• W3204477323 cites W2067216566 @default.
• W3204477323 cites W2069707457 @default.
• W3204477323 cites W2071755982 @default.
• W3204477323 cites W2082369787 @default.
• W3204477323 cites W2104928971 @default.
• W3204477323 cites W2104934425 @default.
• W3204477323 cites W2107551122 @default.
• W3204477323 cites W2111797194 @default.
• W3204477323 cites W2112768620 @default.
• W3204477323 cites W2114487151 @default.
• W3204477323 cites W2115959782 @default.
• W3204477323 cites W2119322011 @default.
• W3204477323 cites W2128870032 @default.
• W3204477323 cites W2142129808 @default.
• W3204477323 cites W2144131446 @default.
• W3204477323 cites W2148658865 @default.
• W3204477323 cites W2153499793 @default.
• W3204477323 cites W2154933939 @default.
• W3204477323 cites W2159269876 @default.
• W3204477323 cites W2164805416 @default.
• W3204477323 cites W2301131921 @default.
• W3204477323 cites W2414734864 @default.
• W3204477323 cites W2466058746 @default.
• W3204477323 cites W2550736836 @default.
• W3204477323 cites W2610184382 @default.
• W3204477323 cites W2611615951 @default.
• W3204477323 cites W2622055116 @default.
• W3204477323 cites W2761986774 @default.
• W3204477323 cites W2763973319 @default.
• W3204477323 cites W2768858469 @default.
• W3204477323 cites W2805692480 @default.
• W3204477323 cites W2885558416 @default.
• W3204477323 cites W2889858315 @default.
• W3204477323 cites W3042705689 @default.
• W3204477323 doi "https://doi.org/10.1165/rcmb.2020-0410oc" @default.
• W3204477323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34597246" @default.
• W3204477323 hasPublicationYear "2022" @default.
• W3204477323 type Work @default.
• W3204477323 sameAs 3204477323 @default.
• W3204477323 citedByCount "13" @default.
• W3204477323 countsByYear W32044773232021 @default.
• W3204477323 countsByYear W32044773232022 @default.
• W3204477323 countsByYear W32044773232023 @default.
• W3204477323 crossrefType "journal-article" @default.
• W3204477323 hasAuthorship W3204477323A5017217713 @default.
• W3204477323 hasAuthorship W3204477323A5021664553 @default.
• W3204477323 hasAuthorship W3204477323A5021974562 @default.
• W3204477323 hasAuthorship W3204477323A5044002777 @default.
• W3204477323 hasAuthorship W3204477323A5073196705 @default.
• W3204477323 hasAuthorship W3204477323A5083435207 @default.
• W3204477323 hasBestOaLocation W32044773232 @default.
• W3204477323 hasConcept C134897094 @default.
• W3204477323 hasConcept C150903083 @default.
• W3204477323 hasConcept C153911025 @default.
• W3204477323 hasConcept C185592680 @default.
• W3204477323 hasConcept C202751555 @default.
• W3204477323 hasConcept C203014093 @default.
• W3204477323 hasConcept C207001950 @default.
• W3204477323 hasConcept C2776317360 @default.
• W3204477323 hasConcept C2776914184 @default.
• W3204477323 hasConcept C2778012055 @default.
• W3204477323 hasConcept C2779244956 @default.
• W3204477323 hasConcept C28406088 @default.
• W3204477323 hasConcept C55493867 @default.
• W3204477323 hasConcept C79879829 @default.
• W3204477323 hasConcept C86803240 @default.
• W3204477323 hasConcept C95444343 @default.
• W3204477323 hasConceptScore W3204477323C134897094 @default.
• W3204477323 hasConceptScore W3204477323C150903083 @default.
• W3204477323 hasConceptScore W3204477323C153911025 @default.
• W3204477323 hasConceptScore W3204477323C185592680 @default.
• W3204477323 hasConceptScore W3204477323C202751555 @default.
• W3204477323 hasConceptScore W3204477323C203014093 @default.

• next