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- W3204512658 abstract "Genome-wide DNA methylation sequencing provides plenty of differentially methylated regions for further implementation with MSRE-qPCR. Multiplex design is challenging requiring unified PCR conditions for all primers/probes, high specificity for target regions and inclusion of MSRE recognition sites. To address these requirements, we developed a method to design multiplex panels of amplicons, and to estimate their diagnostic potential. We have applied this method to our genome-wide DNA methylation XmaI-RRBS data for triple-negative breast cancer (TNBC) to predict response to neoadjuvant chemotherapy (NAC)." @default.
- W3204512658 created "2021-10-11" @default.
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- W3204512658 date "2021-09-01" @default.
- W3204512658 modified "2023-10-14" @default.
- W3204512658 title "1143P Virtual amplicons for methylation-sensitive restriction enzyme quantitative PCR (MSRE-qPCR) derived from genome-wide DNA methylation sequencing: Application to prediction of breast cancer neoadjuvant chemotherapy response" @default.
- W3204512658 doi "https://doi.org/10.1016/j.annonc.2021.08.784" @default.
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