FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204538462> ?p ?o ?g. }

• W3204538462 endingPage "114666" @default.
• W3204538462 startingPage "114666" @default.
• W3204538462 abstract "Ervatamia coronaria, a popular garden plant in India and some other parts of the world is known traditionally for its anti-inflammatory and anti-cancer properties. The molecular bases of these functions remain poorly understood.Efficacies of the existing therapies for colorectal cancer (CRC) are limited by their life-threatening side effects and unaffordability. Therefore, identifying a safer, efficient, and affordable therapeutic is urgent. We studied the anti-CRC activity of an alkaloid-rich fraction of E. coronaria leaf extracts (AFE) and associated underlying mechanism.Activity guided solvant fractionation was adopted to identify the activity in AFE. Different cell lines, and tumor grown in syngeneic mice were used to understand the anti-CRC effect. Methodologies such as LCMS, MTT, RT-qPCR, immunoblot, immunohistochemistry were employed to understand the molecular basis of its activity.We showed that AFE, which carries about six major compounds, is highly toxic to colorectal cancer (CRC) cells. AFE induced cell cycle arrest at G1 phase and p21 and p27 genes, while those of CDK2, CDK-4, cyclin-D, and cyclin-E genes were downregulated in HCT116 cells. It predominantly induced apoptosis in HCT116p53+/+ cells while the HCT116p53-/- cells under the same treatment condition died by autophagy. Notably, AFE induced upregulation of AMPK phosphorylation, and inhibition of both of the mTOR complexes as indicated by inhibition of phosphorylation of S6K1, 4EBP1, and AKT. Furthermore, AFE inhibited mTOR-driven conversion of cells from reversible cell cycle arrest to senescence (geroconversion) as well as ERK activity. AFE activity was independent of ROS produced, and did not primarily target the cellular DNA or cytoskeleton. AFE also efficiently regressed CT26-derived solid tumor in Balb/c mice acting alone or in synergy with 5FU through inducing autophagy as a major mechanism of action as indicated by upregulation of Beclin 1 and phospho-AMPK, and inhibition of phospho-S6K1 levels in the tumor tissue lysates.AFE induced CRC death through activation of both apoptotic and autophagy pathways without affecting the normal cells. This study provided a logical basis for consideration of AFE in future therapy regimen to overcome the limitations associated with existing anti-CRC chemotherapy." @default.
• W3204538462 created "2021-10-11" @default.
• W3204538462 creator A5006358762 @default.
• W3204538462 creator A5015550222 @default.
• W3204538462 creator A5016686272 @default.
• W3204538462 creator A5025951772 @default.
• W3204538462 creator A5039097008 @default.
• W3204538462 creator A5041161409 @default.
• W3204538462 creator A5042042567 @default.
• W3204538462 creator A5050031621 @default.
• W3204538462 creator A5055098716 @default.
• W3204538462 creator A5057820435 @default.
• W3204538462 creator A5069808553 @default.
• W3204538462 creator A5075528445 @default.
• W3204538462 creator A5076725351 @default.
• W3204538462 creator A5083448314 @default.
• W3204538462 creator A5084057504 @default.
• W3204538462 date "2022-01-01" @default.
• W3204538462 modified "2023-09-27" @default.
• W3204538462 title "Alkaloid-rich fraction of Ervatamia coronaria sensitizes colorectal cancer through modulating AMPK and mTOR signalling pathways" @default.
• W3204538462 cites W1193385461 @default.
• W3204538462 cites W1963602315 @default.
• W3204538462 cites W1965694039 @default.
• W3204538462 cites W1967422586 @default.
• W3204538462 cites W1968075505 @default.
• W3204538462 cites W1974253337 @default.
• W3204538462 cites W1979783084 @default.
• W3204538462 cites W1985775217 @default.
• W3204538462 cites W1987460060 @default.
• W3204538462 cites W1987515837 @default.
• W3204538462 cites W1987702513 @default.
• W3204538462 cites W1990532502 @default.
• W3204538462 cites W2001568034 @default.
• W3204538462 cites W2028304108 @default.
• W3204538462 cites W2043263760 @default.
• W3204538462 cites W2066125776 @default.
• W3204538462 cites W2071251338 @default.
• W3204538462 cites W2078765043 @default.
• W3204538462 cites W2078821200 @default.
• W3204538462 cites W2079580613 @default.
• W3204538462 cites W2081920342 @default.
• W3204538462 cites W2089917418 @default.
• W3204538462 cites W2115957809 @default.
• W3204538462 cites W2144600779 @default.
• W3204538462 cites W2160615487 @default.
• W3204538462 cites W2174804804 @default.
• W3204538462 cites W2334852544 @default.
• W3204538462 cites W2517175971 @default.
• W3204538462 cites W2571999091 @default.
• W3204538462 cites W2581841075 @default.
• W3204538462 cites W2593816418 @default.
• W3204538462 cites W2737292778 @default.
• W3204538462 cites W2763871199 @default.
• W3204538462 cites W2783037283 @default.
• W3204538462 cites W2793589490 @default.
• W3204538462 cites W2860743873 @default.
• W3204538462 cites W2884499015 @default.
• W3204538462 cites W2890156259 @default.
• W3204538462 cites W2897974793 @default.
• W3204538462 cites W2995338836 @default.
• W3204538462 cites W3113723146 @default.
• W3204538462 cites W3130119312 @default.
• W3204538462 cites W3131881882 @default.
• W3204538462 cites W3157865444 @default.
• W3204538462 cites W3164057582 @default.
• W3204538462 cites W3165525425 @default.
• W3204538462 cites W3169464055 @default.
• W3204538462 cites W4211199921 @default.
• W3204538462 cites W1984759161 @default.
• W3204538462 doi "https://doi.org/10.1016/j.jep.2021.114666" @default.
• W3204538462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34592338" @default.
• W3204538462 hasPublicationYear "2022" @default.
• W3204538462 type Work @default.
• W3204538462 sameAs 3204538462 @default.
• W3204538462 citedByCount "7" @default.
• W3204538462 countsByYear W32045384622022 @default.
• W3204538462 countsByYear W32045384622023 @default.
• W3204538462 crossrefType "journal-article" @default.
• W3204538462 hasAuthorship W3204538462A5006358762 @default.
• W3204538462 hasAuthorship W3204538462A5015550222 @default.
• W3204538462 hasAuthorship W3204538462A5016686272 @default.
• W3204538462 hasAuthorship W3204538462A5025951772 @default.
• W3204538462 hasAuthorship W3204538462A5039097008 @default.
• W3204538462 hasAuthorship W3204538462A5041161409 @default.
• W3204538462 hasAuthorship W3204538462A5042042567 @default.
• W3204538462 hasAuthorship W3204538462A5050031621 @default.
• W3204538462 hasAuthorship W3204538462A5055098716 @default.
• W3204538462 hasAuthorship W3204538462A5057820435 @default.
• W3204538462 hasAuthorship W3204538462A5069808553 @default.
• W3204538462 hasAuthorship W3204538462A5075528445 @default.
• W3204538462 hasAuthorship W3204538462A5076725351 @default.
• W3204538462 hasAuthorship W3204538462A5083448314 @default.
• W3204538462 hasAuthorship W3204538462A5084057504 @default.
• W3204538462 hasConcept C104202773 @default.
• W3204538462 hasConcept C105696609 @default.
• W3204538462 hasConcept C124320809 @default.
• W3204538462 hasConcept C184235292 @default.
• W3204538462 hasConcept C185592680 @default.

• next