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- W3204602317 abstract "Abstract Amphiphilic copolymers show great promise in extracting membrane proteins directly from lipid bilayers into ‘native nanodiscs’. However, many such copolymers are polyanionic and sensitive to divalent cations, limiting their applicability towards Ca 2+ or Mg 2+ dependent proteins. Here, we characterize the Ca 2+ and Mg 2+ sensitivity of poly(acrylic acid-co-styrene) (AASTY) copolymers using analytical UV and fluo-rescent size exclusion chromatography, enabling us to separate signals from nanodiscs, copolymers, and soluble aggregates. Determination of free Ca 2+ ion concentrations in the presence of copolymer shows that divalent cation tolerance is dependent on not only specific characteristics of a copolymer, but also on its concentration. We see that high ionic strength protects against aggregation facilitated by divalent cations, which is prominent in nanodiscs isolated from excess free copolymer through dialysis. Overall, we conclude that the behavior of amphiphilic copolymers in the presence of divalent cations is more complex than precipitation beyond a specific cation concentration." @default.
- W3204602317 created "2021-10-11" @default.
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- W3204602317 date "2021-10-07" @default.
- W3204602317 modified "2023-09-26" @default.
- W3204602317 title "Characterization of divalent cation interactions with AASTY nanodiscs" @default.
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- W3204602317 doi "https://doi.org/10.1101/2021.10.07.463511" @default.
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