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- W3204604614 abstract "In this article, we have shown the results of E. coli bacteria determination in water using the enzyme free electrochemical immunosensor with covalent via click chemistry immobilized receptor layer in comparison with dropwise immobilized antibodies. Covalent immobilization was realized by creating precursors on the surface of the working electrode with the reaction of azide-alkyne copper-catalysed cycloaddition approach. The detection limit of the immunosensor with covalent and dropwise immobilization of antibodies was estimated as 6.6 CFU/ml and 11.2 CFU/ml, respectively, a linear range was 103-106 CFU/ml. Moreover, the sensor with click immobilized antibodies showed good stability for 30 days when stored in a phosphate buffer, while the sensor with dropwise immobilized receptor layer was stable for 3 days." @default.
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- W3204604614 date "2021-09-01" @default.
- W3204604614 modified "2023-10-18" @default.
- W3204604614 title "Determination of E. coli in water using the enzyme free electrochemical impedimetric immunosensors" @default.
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- W3204604614 doi "https://doi.org/10.1088/1755-1315/864/1/012017" @default.
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