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- W3204646884 endingPage "10667" @default.
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- W3204646884 abstract "Background: Mesenchymal stromal cells (MSCs) have the capacity for self-renewal and multi-differentiation, and for this reason they are considered a potential cellular source in regenerative medicine of cartilage and bone. However, research on this field is impaired by the predisposition of primary MSCs to senescence during culture expansion. Therefore, the aim of this study was to generate and characterize immortalized MSC (iMSC) lines from aged donors. Methods: Primary MSCs were immortalized by transduction of simian virus 40 large T antigen (SV40LT) and human telomerase reverse transcriptase (hTERT). Proliferation, senescence, phenotype and multi-differentiation potential of the resulting iMSC lines were analyzed. Results: MSCs proliferate faster than primary MSCs, overcome senescence and are phenotypically similar to primary MSCs. Nevertheless, their multi-differentiation potential is unbalanced towards the osteogenic lineage. There are no clear differences between osteoarthritis (OA) and non-OA iMSCs in terms of proliferation, senescence, phenotype or differentiation potential. Conclusions: Primary MSCs obtained from elderly patients can be immortalized by transduction of SV40LT and hTERT. The high osteogenic potential of iMSCs converts them into an excellent cellular source to take part in in vitro models to study bone tissue engineering." @default.
- W3204646884 created "2021-10-11" @default.
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- W3204646884 date "2021-10-01" @default.
- W3204646884 modified "2023-10-15" @default.
- W3204646884 title "Generation of Mesenchymal Cell Lines Derived from Aged Donors" @default.
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- W3204646884 doi "https://doi.org/10.3390/ijms221910667" @default.
- W3204646884 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8508916" @default.
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- W3204646884 hasPublicationYear "2021" @default.
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