Matches in SemOpenAlex for { <https://semopenalex.org/work/W3204666530> ?p ?o ?g. }
- W3204666530 abstract "Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated bacterial infections. This strain has a unique genetic code, which hinders gene transfer to most other bacterial genera, and it lacks a cell wall, which allows it to express proteins that target peptidoglycans of pathogenic bacteria. We first determined that removal of the pathogenic factors fully attenuated the chassis strain in vivo. We then designed synthetic promoters and identified an endogenous peptide signal sequence that, when fused to heterologous proteins, promotes efficient secretion. Based on this, we equipped the chassis strain with a genetic platform designed to secrete antibiofilm and bactericidal enzymes, resulting in a strain capable of dissolving Staphylococcus aureus biofilms preformed on catheters in vitro, ex vivo, and in vivo. To our knowledge, this is the first engineered genome-reduced bacterium that can fight against clinically relevant biofilm-associated bacterial infections." @default.
- W3204666530 created "2021-10-11" @default.
- W3204666530 creator A5004529434 @default.
- W3204666530 creator A5005061862 @default.
- W3204666530 creator A5007339532 @default.
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- W3204666530 creator A5050208790 @default.
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- W3204666530 creator A5065953954 @default.
- W3204666530 creator A5068857327 @default.
- W3204666530 creator A5080707475 @default.
- W3204666530 date "2021-10-01" @default.
- W3204666530 modified "2023-10-14" @default.
- W3204666530 title "Engineering a genome‐reduced bacterium to eliminate<i>Staphylococcus aureus</i>biofilms<i>in vivo</i>" @default.
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