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• W3204692534 abstract "The dynamics of living organisms are organized across many spatial scales. However, current cost-effective imaging systems can measure only a subset of these scales at once. We have created a scalable multi-camera array microscope (MCAM) that enables comprehensive high-resolution recording from multiple spatial scales simultaneously, ranging from cellular structures to large-group behavioral dynamics. By collecting data from up to 96 cameras, we computationally generate gigapixel-scale images and movies with a field of view over hundreds of square centimeters at 5um sensitivity. This allows us to observe the behavior and fine anatomical features of numerous freely moving model organisms on multiple spatial scales, including larval zebrafish, fruit flies, nematodes, carpenter ants, and slime mold. The MCAM architecture allows stereoscopic tracking of the z-position of organisms using the overlapping field of view from adjacent cameras. Further, we demonstrate the ability to acquire dual color fluorescence video of multiple freely moving zebrafish, recording neural activity via ratiometric calcium imaging. Overall, the MCAM provides a powerful platform for investigating cellular and behavioral processes across a wide range of spatial scales by removing the bottlenecks imposed by single-camera image acquisition systems." @default.
• W3204692534 created "2021-10-11" @default.
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• W3204692534 date "2021-10-06" @default.
• W3204692534 modified "2023-10-14" @default.
• W3204692534 title "Gigapixel behavioral and neural activity imaging with a novel multi-camera array microscope" @default.
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• W3204692534 doi "https://doi.org/10.1101/2021.10.05.461695" @default.
• W3204692534 hasPublicationYear "2021" @default.
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