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• W3204696491 abstract "<p>Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - the peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.<i></i></p>" @default.
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• W3204696491 date "2020-02-11" @default.
• W3204696491 modified "2023-09-24" @default.
• W3204696491 title "Synthesis of Proteins by Automated Flow Chemistry" @default.
• W3204696491 doi "https://doi.org/10.26434/chemrxiv.11833503.v1" @default.
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