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• W3204722153 abstract "Abstract Introduction Toxoplasma gondii is one of the most prevalent zoonotic protozoan parasites worldwide and affects the vast majority of warm-blooded animal species, including humans. Postnatal infection in humans occurs through the ingestion of sporulated T. gondii oocysts or via the oral intake of parasite tissue cysts during the consumption of raw or undercooked meat. In this regard, given their high exposure to oocysts, chickens ( Gallus domesticus ) raised on the ground constitute a potential source of T. gondii . Material and Methods For the first time in Spain, a survey was undertaken in commercial retail free-range poultry. A total of 50 thighs from different animals were analysed. The samples were homogenised and an acid pepsin digestion procedure was applied prior to molecular analysis. Toxoplasma gondii DNA was isolated from meat by qPCR. Two sets of primers were used for DNA amplification targeting the specific sequence of a 529 bp repeat element and another set of primers was utilised for the surface antigen protein-1 gene. Results DNA extracted from 5 out of 50 tissue samples was positive for both genes by qPCR amplification. Conclusion The 10% prevalence of Toxoplasma infection found in commercial free-range chickens raises public health issues." @default.
• W3204722153 created "2021-10-11" @default.
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• W3204722153 date "2021-10-06" @default.
• W3204722153 modified "2023-10-03" @default.
• W3204722153 title "Prevalence of <i>Toxoplasma gondii</i> in retail fresh meat products from free-range chickens in Spain" @default.
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• W3204722153 doi "https://doi.org/10.2478/jvetres-2021-0052" @default.
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