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• W3204754937 abstract "The objective of this study was to evaluate the effect of dry matter (DM) concentration and the use of lactic acid bacteria (LAB) inoculant on the protein value of grass silages. Grass samples containing 170 g DM/kg fresh material were wilted to different DM concentrations (310, 390, 420, 470, 530, 580, 600 g/kg fresh material) before ensiling. Half of the grass material at respective DM was treated with LAB, the other half remained untreated (control, CON). Ruminal degradation of organic matter (OM) and crude protein (CP) was determined using the in situ technique. For this, silage samples were incubated for 2, 4, 8, 16, 24, 48 and 72 h. Rumen undegraded CP (RUCP) was derived from in situ CP degradation. Microbial CP (MCP) synthesis was assumed to be 181 g per kg fermented OM. Post-ruminal CP (prCP) was defined as the sum of RUCP and MCP. Effective prCP in situ (prCPk in situ) was calculated for passage rates (k) of 0.02, 0.04 and 0.06/h. In addition, the modified Hohenheim gas test (modHGT) was used to determine effective prCP in vitro (prCPk in vitro) for the same passage rates. The DM linearly increased prCPk in situ in CON (P < 0.001) and LAB (P < 0.001). This was caused by both increased RUCP in the range of 580–600 g DM (P < 0.001) and increased MCP (P < 0.001) due to higher amount of water-soluble carbohydrates in the more intensively wilted silages. The use of LAB increased RUCP (P < 0.001) but decreased the calculated MCP content (P < 0.001). Therefore, these effects compensated each other leading to only slightly lower prCPk in situ for LAB (P < 0.05). The positive effect of DM on prCPk in situ was confirmed in vitro (P < 0.001) showing no effect of the inoculant. Based on both methods prCPk increased to a small extent with 2.3 – 4.9 g/kg OM (P < 0.001), when DM increases by 100 g/kg fresh material, depending on the assumed passage rate. Therefore, wilting and LAB addition to grass have the potential to improve protein value of grass silage. Both methods delivered similar estimates for effective prCP. But modHGT appeared to be superior to the in situ technique by being more sensitive, not requiring separate estimation of MCP and the assumption of a certain efficiency value, and by having lower operating expenses. The results of this study show the potential of the modHGT, however further studies are required to standardize and validate this technique and to evaluate the effect of wilting grass on animal performance." @default.
• W3204754937 created "2021-10-11" @default.
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• W3204754937 date "2021-12-01" @default.
• W3204754937 modified "2023-09-26" @default.
• W3204754937 title "Effect of wilting and lactic acid bacteria inoculant on in situ and in vitro determined protein value of grass silages" @default.
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• W3204754937 doi "https://doi.org/10.1016/j.anifeedsci.2021.115115" @default.
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