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• W3204806057 abstract "We have developed a rapid, inexpensive, colorimetric and easy to use method able to detect living bacterial pathogens of zoonotic and foodborne interest. The method is based on detection of bacterial nucleases which cut selectively oligonucleotide probes that are designed in a way to induce aggregation of oligonucleotide stabilised gold-nanoparticles. We present the standardization of our method to detect nucleases secreted by Gram-positive ( Staphylococcus aureus ) and Gram-negative ( Salmonella spp.) pathogens, not only in five different matrixes of food samples experimentally contaminated but also in naturally contaminated foodstuffs. Our method has shown sensitivity/specificity, detecting nucleases in less than two hours in supernatants from bacterial cell culture (1 CFU/mL) incubated for 15 h. The nucleases are detected by naked-eye inspection, and using minimal laboratory equipment. From a broader perspective, besides applications in foodstuff safety, we envision a potential use of our method to detect other bacterial and viral pathogens in the environment, and in veterinary and human health. • The presence of nucleases secreted by living bacteria is detected by the DNA-functionalised gold nanoparticles. • A nuclease, by fragmenting the DNA bonds, prevents aggregation of the nanoparticles, giving a red colour to the solution as a positive result. • The assay detects nucleases secreted by S. aureus , S. Typhimurium and S. Enteritidis up to 1 CFU/mL. • The presence of nucleases in naturally contaminated food samples is detected in less than two hours." @default.
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• W3204806057 date "2021-12-01" @default.
• W3204806057 modified "2023-10-17" @default.
• W3204806057 title "Plasmon-assisted fast colorimetric detection of bacterial nucleases in food samples" @default.
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• W3204806057 doi "https://doi.org/10.1016/j.snb.2021.130780" @default.
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