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• W3204899257 abstract "Yeast morphology and counting are highly important in fermentation as they are often associated with productivity and can be influenced by process conditions. At present, time-consuming and offline methods are utilized for routine analysis of yeast morphology and cell counting using a haemocytometer. In this study, we demonstrate the application of an in situ microscope to obtain a fast stream of pseudohyphae images from agitated sample suspensions of a Saccharomyces cerevisiae strain, whose morphology in cell clusters is frequently found in the bioethanol fermentation industry. The large statistics of microscopic images allow for online determination of the principal morphological characteristics of the pseudohyphae, including the number of constituent cells, cell-size, number of branches, and length of branches. The distributions of these feature values are calculated online, constituting morphometric monitoring of the pseudohyphae population. By providing representative data, the proposed system can improve the effectiveness of morphological characterization, which in turn can help to improve the understanding and control of bioprocesses in which pseudohyphal-like morphologies are found. • First application of in situ microscopy to pseudohyphal yeast suspensions. • An image analysis algorithm for morphometric quantification was developed. • Quick and reliable enumeration of constituent cells in pseudohyphae can be attained." @default.
• W3204899257 created "2021-10-11" @default.
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• W3204899257 date "2021-11-01" @default.
• W3204899257 modified "2023-09-27" @default.
• W3204899257 title "Morphometric quantification of a pseudohyphae forming Saccharomyces cerevisiae strain using in situ microscopy and image analysis" @default.
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• W3204899257 doi "https://doi.org/10.1016/j.mimet.2021.106338" @default.
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