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• W3205062949 abstract "Establishing personalized medication plans for patients to maximize therapeutic efficacy and minimize the toxicity of vancomycin (VAN) requires rapid, simple, and accurate monitoring of VAN concentration in body fluid. In this study, we have developed a simple and rapid analytical method by integrating Eu (III) chelate nanoparticles (CN-EUs) and lateral flow immunoassay (LFIA) to achieve the real-time monitoring of VAN concentration in serum within 15 min. This approach was performed on nitrocellulose (NC) membrane assembled LFIA strips via indirect competitive immunoassay and exhibited a wide linear range of detection (0.1-80 μg*ml-1) with a low limit of detection (69.2 ng*ml-1). The coefficients of variation (CV) of the intra- and inter-assay in the detection of VAN were 7.12-8.53% and 8.46-11.82%, respectively. The dilution test and specificity indicated this method had a stability that was not affected by the serum matrix and some other antibiotics. Furthermore, the applicability of the proposed method was assessed by comparing the determined results with those measured by LC-MS/MS, showing a satisfactory correlation (R2 = 0.9713). The proposed CN-EUs-based LFIA manifested promising analytical performance, which showed potential value in the real-time monitoring of VAN and could help optimize the clinical use of more antibiotics." @default.
• W3205062949 created "2021-10-25" @default.
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• W3205062949 date "2021-10-18" @default.
• W3205062949 modified "2023-10-11" @default.
• W3205062949 title "Rapid Monitoring of Vancomycin Concentration in Serum Using Europium (III) Chelate Nanoparticle-Based Lateral Flow Immunoassay" @default.
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• W3205062949 doi "https://doi.org/10.3389/fchem.2021.763686" @default.
• W3205062949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8558538" @default.
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