Matches in SemOpenAlex for { <https://semopenalex.org/work/W3205113417> ?p ?o ?g. }
- W3205113417 endingPage "109853" @default.
- W3205113417 startingPage "109853" @default.
- W3205113417 abstract "Currently, many genetic methods are available for mapping chemical connectivity, but analogous methods for electrical synapses are lacking. Here, we present pupylation-based interaction labeling (PUPIL), a genetically encoded system for noninvasively mapping and stamping transient electrical synapses in the mouse brain. Upon fusion of connexin 26 (CX26) with the ligase PafA, pupylation yields tag puncta following conjugation of its substrate, a biotin- or fluorescent-protein-tagged PupE, to the neighboring proteins of electrical synapses containing CX26-PafA. Tag puncta are validated to correlate well with functional electrical synapses in immature neurons. Furthermore, puncta are retained in mature neurons when electrical synapses mostly disappear-suggesting successful stamping. We use PUPIL to uncover spatial subcellular localizations of electrical synapses and approach their physiological functions during development. Thus, PUPIL is a powerful tool for probing electrical connectivity patterns in complex nervous systems and has great potential for transient receptors and ion channels as well." @default.
- W3205113417 created "2021-10-25" @default.
- W3205113417 creator A5015357427 @default.
- W3205113417 creator A5019938190 @default.
- W3205113417 creator A5020582038 @default.
- W3205113417 creator A5028462736 @default.
- W3205113417 creator A5030757374 @default.
- W3205113417 creator A5034704065 @default.
- W3205113417 creator A5054320636 @default.
- W3205113417 creator A5057055806 @default.
- W3205113417 creator A5060440099 @default.
- W3205113417 date "2021-10-01" @default.
- W3205113417 modified "2023-10-06" @default.
- W3205113417 title "PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems" @default.
- W3205113417 cites W1489513029 @default.
- W3205113417 cites W1513082520 @default.
- W3205113417 cites W1624401920 @default.
- W3205113417 cites W1925508137 @default.
- W3205113417 cites W1942860519 @default.
- W3205113417 cites W1975173458 @default.
- W3205113417 cites W1976103584 @default.
- W3205113417 cites W1977691361 @default.
- W3205113417 cites W1991147662 @default.
- W3205113417 cites W1996761102 @default.
- W3205113417 cites W1997122736 @default.
- W3205113417 cites W2001453133 @default.
- W3205113417 cites W2017196830 @default.
- W3205113417 cites W2019534872 @default.
- W3205113417 cites W2022045077 @default.
- W3205113417 cites W2022235653 @default.
- W3205113417 cites W2025603247 @default.
- W3205113417 cites W2034424864 @default.
- W3205113417 cites W2037820041 @default.
- W3205113417 cites W2067043311 @default.
- W3205113417 cites W2080670791 @default.
- W3205113417 cites W2081368671 @default.
- W3205113417 cites W2081444617 @default.
- W3205113417 cites W2081669905 @default.
- W3205113417 cites W2081918193 @default.
- W3205113417 cites W2085261999 @default.
- W3205113417 cites W2087849583 @default.
- W3205113417 cites W2088081549 @default.
- W3205113417 cites W2092064796 @default.
- W3205113417 cites W2099021265 @default.
- W3205113417 cites W2099321759 @default.
- W3205113417 cites W2099401565 @default.
- W3205113417 cites W2103019017 @default.
- W3205113417 cites W2112955454 @default.
- W3205113417 cites W2114538750 @default.
- W3205113417 cites W2124789276 @default.
- W3205113417 cites W2130546860 @default.
- W3205113417 cites W2164826784 @default.
- W3205113417 cites W2226944184 @default.
- W3205113417 cites W2261497167 @default.
- W3205113417 cites W2512022815 @default.
- W3205113417 cites W2593319303 @default.
- W3205113417 cites W2765238105 @default.
- W3205113417 cites W2810169789 @default.
- W3205113417 cites W2887898165 @default.
- W3205113417 cites W2950361439 @default.
- W3205113417 cites W2982268628 @default.
- W3205113417 cites W4211091257 @default.
- W3205113417 doi "https://doi.org/10.1016/j.celrep.2021.109853" @default.
- W3205113417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34686323" @default.
- W3205113417 hasPublicationYear "2021" @default.
- W3205113417 type Work @default.
- W3205113417 sameAs 3205113417 @default.
- W3205113417 citedByCount "6" @default.
- W3205113417 countsByYear W32051134172021 @default.
- W3205113417 countsByYear W32051134172022 @default.
- W3205113417 countsByYear W32051134172023 @default.
- W3205113417 crossrefType "journal-article" @default.
- W3205113417 hasAuthorship W3205113417A5015357427 @default.
- W3205113417 hasAuthorship W3205113417A5019938190 @default.
- W3205113417 hasAuthorship W3205113417A5020582038 @default.
- W3205113417 hasAuthorship W3205113417A5028462736 @default.
- W3205113417 hasAuthorship W3205113417A5030757374 @default.
- W3205113417 hasAuthorship W3205113417A5034704065 @default.
- W3205113417 hasAuthorship W3205113417A5054320636 @default.
- W3205113417 hasAuthorship W3205113417A5057055806 @default.
- W3205113417 hasAuthorship W3205113417A5060440099 @default.
- W3205113417 hasBestOaLocation W32051134171 @default.
- W3205113417 hasConcept C127445978 @default.
- W3205113417 hasConcept C158157758 @default.
- W3205113417 hasConcept C169760540 @default.
- W3205113417 hasConcept C2780228694 @default.
- W3205113417 hasConcept C2908872077 @default.
- W3205113417 hasConcept C545706735 @default.
- W3205113417 hasConcept C79879829 @default.
- W3205113417 hasConcept C86803240 @default.
- W3205113417 hasConcept C95444343 @default.
- W3205113417 hasConceptScore W3205113417C127445978 @default.
- W3205113417 hasConceptScore W3205113417C158157758 @default.
- W3205113417 hasConceptScore W3205113417C169760540 @default.
- W3205113417 hasConceptScore W3205113417C2780228694 @default.
- W3205113417 hasConceptScore W3205113417C2908872077 @default.
- W3205113417 hasConceptScore W3205113417C545706735 @default.
- W3205113417 hasConceptScore W3205113417C79879829 @default.